Digoxigenin Antibody (PSH04-39)

Western Blot: Digoxigenin Antibody (PSH04-39) [NBP3-32266]

Western Blot: Digoxigenin Antibody (PSH04-39) [NBP3-32266] - Western blot analysis of Digoxigenin on different lysates with Rabbit anti-Digoxigenin antibody (NBP3-32266) at 1/2,000 dilution.

Lane 1: DIG-BSA (2 ng/Lane)
Lane 2: BSA (negative) (2 ng/Lane)
Lane 3: 293T cell lysate (negative) (20 ug/Lane)

Exposure time: 20 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32266) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Digoxigenin Antibody (PSH04-39) [NBP3-32266]

Immunohistochemistry: Digoxigenin Antibody (PSH04-39) [NBP3-32266] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue (negative) with Rabbit anti-Digoxigenin antibody (NBP3-32266) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32266) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.