Disulfiram

Tocris Bioscience | Catalog # 3807

Reversibly stimulates SERCA Ca2+-ATPase; displays a range of other activities
Tocris Bioscience

Key Product Details

Description

Reversibly stimulates SERCA Ca2+-ATPase; displays a range of other activities

Alternative Names

Tetraethylthiuram disulfide
View all Ca2+-ATPase Activators »

Product Description

Disulfiram is an inhibitor of aldehyde dehydrogenase that displays antialcoholism activity. Shown to reversibly stimulate Ca2+-ATPase activity and inhibit V-ATPase (EC50 = 26 μM). Also inhibits expression of MMP-2 and MMP-9 and displays anti-invasive activity. Pyroptosis inhibitor; binds gasdermin D (GSDMD) and inhibits pore formation. Also inhibits SARS-CoV-2 main protease (Mpro; IC50 = 9.35 μM) and inhibits viral replication in Vero cells in vitro.

Product Specifications for Disulfiram

Molecular Weight

296.54

Formula

C10H20N2S4

Storage

Store at +4°C

Chemical Name

Bis(diethylthiocarbamyoyl)disulfide

CAS Number

97-77-8

PubChem ID

3117

InChI Key

AUZONCFQVSMFAP-UHFFFAOYSA-N

SMILES

S=C(SSC(N(CC)CC)=S)N(CC)CC

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 5.93 20
Ethanol 29.65 100

Preparing Stock Solutions for Disulfiram

The following data is based on the product molecular weight 296.54.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.37 mL 16.86 mL 33.72 mL
5 mM 0.67 mL 3.37 mL 6.74 mL
10 mM 0.34 mL 1.69 mL 3.37 mL
50 mM 0.07 mL 0.34 mL 0.67 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 21 publications citing the usage of this product.

Product Documents for Disulfiram

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Disulfiram

For research use only

Related Research Areas

Ion Channels and Regulators

Customer Reviews for Disulfiram (1)

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  • Irreversible inhibitor of Aldh1 with IC50 initial at 50 uM
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: recombinant mouse Aldh1
    Verified Customer | Posted 10/26/2019
    Pre-incubation of recombinant enzyme will lead to full depletion of activity. Observed IC50 (initial) at approximately 50 uM.
    Recombinant Aldh1 was used in a standard fluorometric reporter assay with NAD+ as a cofactor, reaction velocity measured as relative changes in fluorescence. Disulfiram was added immediately prior to assessing activity. We noted decreasing velocity rates over time supporting this as a time-dependent/ irreversible inhibitor of Aldh enzymes.
    Disulfiram 3807

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