Rat Dkk-2 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-82492

Novus Biologicals
100% Guarantee

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.09 IU/mL

Assay Range

0.16 - 10IU/mL
View all Dkk-2 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Specificity

This kit recognizes Rat Dkk-2 in samples. No Significant cross-reactivity or interference between Rat Dkk-2 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 8.25

Inter-Assay Precision (Precision between assays) CV% 10.46

Recovery for Rat Dkk-2 ELISA Kit (Colorimetric)

Recovery

87-97%

Linearity

Rat Dkk-2 ELISA Kit (Colorimetric)

Scientific Data Images for Rat Dkk-2 ELISA Kit (Colorimetric)

Rat Dkk-2 ELISA Kit (Colorimetric)

ELISA: Rat Dkk-2 ELISA Kit (Colorimetric) [NBP2-82492] -

ELISA: Rat Dkk-2 ELISA Kit (Colorimetric) [NBP2-82492] - Standard Curve Reference

Kit Contents for Rat Dkk-2 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Dkk-2

Analysis of individual DKK domains and chimeric DKKs shows that the carboxy-terminal domains of both DKK1 and DKK2 associate with LRP6 and are necessary and sufficient for Wnt8 inhibition. Xenopus Dickkopf 1 (DKK1) was initially discovered as a Wnt antagonist that plays an important role in head formation. By far, four members of DKK have been identified in mammals. Each DKK molecule contains two conserved cysteine-rich domains. Recent studies showed that the second Cys-rich domains of DKK1 and DKK2 inhibited Wnt3a-activated signaling, whereas the first Cys-rich domains had no effects. In addition, the second Cys-rich domain of DKK2, but not that of DKK1, was able to activate the canonical pathway in the presence of LRP6, and this LRP-dependent signaling does not require Dvl.

Long Name

Dickkopf-2

Alternate Names

Dkk2

Gene Symbol

DKK2

Additional Dkk-2 Products

Product Documents for Rat Dkk-2 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rat Dkk-2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Customer Reviews for Rat Dkk-2 ELISA Kit (Colorimetric)

There are currently no reviews for this product. Be the first to review Rat Dkk-2 ELISA Kit (Colorimetric) and earn rewards!

Have you used Rat Dkk-2 ELISA Kit (Colorimetric)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for ELISA Kits