(±)-Blebbistatin

Key Product Details

Product Description

(±)-Blebbistatin is a selective inhibitor of myosin II ATPase activity; inhibits contraction of the cleavage furrow without disrupting mitosis or contractile ring assembly. (±)-Blebbistatin rapidly and reversibly blocks cell blebbing, and disrupts directed cell migration and cytokinesis in vertebrate cells. (±)-Blebbistatin inhibits arterial thrombosis development, reduces infiltration of inflammatory cells, and reduces damage to vascular tissue in vivo in a mice model of arterial thrombosis.

Active and Inactive Enantiomer also available.

Product Specifications for (±)-Blebbistatin

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for (±)-Blebbistatin

The following data is based on the product molecular weight 292.34.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

11 10 9 8 7 6 5 4 3 2 1 Recalculate

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	3.42 mL	17.10 mL	34.21 mL
5 mM	0.68 mL	3.42 mL	6.84 mL
10 mM	0.34 mL	1.71 mL	3.42 mL
50 mM	0.07 mL	0.34 mL	0.68 mL

Calculators

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 303 publications citing the usage of this product.

• Wang Myosin IIA-related actomyosin contractility mediates oxidative stress-induced neuronal apoptosis. Front Mol.Neurosci. 2017 PMID:  28352215
• Chen Blebbistatin modulates prostatic cell growth and contrapctility through myosin II signaling. Clin.Sci. 2018 PMID:  30279228
• Gao Blebbistatin inhibits neomycin-induced apoptosis in hair cell-like HEI-OC-1 cells and in cochlear hair cells. Front.Cell Neurosci. 2020 PMID: 32116554
• Lang Inhibition of suicidal erythrocyte death by blebbistatin Am.J.Physiol.Cell Physiol. 2011 PMID: 21593446
• Zhang An inhibitor of myosin II, blebbistatin, suppresses development of arterial thrombosis. Biomed.Pharmacother 2020 PMID:  31918291
• Walker Non-muscle myosin II regulates survival threshold of pluripotent stem cells. Nat.Commun. 2010 PMID: 20842192
• Straight Dissecting cytokinesis with small molecules. Amer.Soc.Cell Biol.Abstr.
• Straight Dissecting temporal and spatial control of cytokinesis with a myosin II inhibitor. Science 2003 PMID: 12637748
• Limouze Specificity of blebbistatin, an inhibitor of myosin II. J.Muscle Res.Cell Motil. 2004 PMID: 15548862

Product Documents for (±)-Blebbistatin