DMC1 Antibody (1D12/4) - BSA Free

Novus Biologicals | Catalog # NB100-181

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1D12/4

Format

BSA Free
View all DMC1 Primary Antibodies »

Product Specifications

Immunogen

This DMC1 Antibody (1D12/4) is made against His-tagged human DMC1, overexpressed in E. coli [UniProt# Q14565].

Reactivity Notes

Does not cross-react with human Rad51 in Western blot analysis.

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DMC1 Antibody (1D12/4) - BSA Free

Western Blot: DMC1 Antibody (1D12/4) [NB100-181]

Western Blot: DMC1 Antibody (1D12/4) [NB100-181]

Western Blot: DMC1 Antibody (1D12/4) [NB100-181] - Analysis of DMC1 on human testis using NB100-181.

Applications for DMC1 Antibody (1D12/4) - BSA Free

Application
Recommended Usage

Immunoprecipitation

reported in scientific literature

Western Blot

1:1000
Application Notes
In Western Blot, a band can be seen at ~37 kDa for endogenous protein and ~40.5 kDa for recombinant his-tagged DMC1. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 1 using NB100-181 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DMC1

DMC1 (disrupted meiotic cDNA or LIM15) is a meiosis-specific gene that encodes a nuclear protein which is essential for meiotic homologous recombination, specifically in homologous strand assimilation, and MDC1 also participates in formation of synaptonemal complex as well as double-strand breaks repair at recombination hotspots. DMC1 is a member of RecA family and in eukaryotic cells; there are two RecA homologs, RAD51 and DMC1, which promote the search for homologous DNA sequences and DNA strand exchange resulting in the formation of joint molecules. RAD51 acts both in meiotic recombinations as well as during DNA repair in mitotically dividing cells, however, DMC1 is expressed only during meiosis only. DMC1 interacts with the MND1-PSMC3IP heterodimer and with BRCA2. Dmc1-/- mice are infertile due to decreased germ cells, failing to form synaptonemal complexes and arresting at the leptotene or zygotene stage in late meiosis I. In female mice lacking Dmc1, normal oogenesis is aborted in embryos, and germ cells are depleted in the adult ovary. The phenotypes of Dmc1-/- ovaries are similar to clinical characteristics found in women with premature ovarian failure (POF), however, mutations in the coding sequence of DMC1 were shown to not to associate with POF in women.

Alternate Names

disrupted meiotic cDNA1, yeast, homolog of, DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologousrecombination (yeast), DMC1 homologue, HsLim15, LIM15yeast homolog) meiosis-specific homologousrecombination, meiotic recombination protein DMC1/LIM15 homolog, MGC150472, MGC150473

Entrez Gene IDs

11144 (Human); 13404 (Mouse)

Gene Symbol

DMC1

UniProt

Q14565 (Human)

Additional DMC1 Products

Product Documents for DMC1 Antibody (1D12/4) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for DMC1 Antibody (1D12/4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DMC1 Antibody (1D12/4) - BSA Free

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  • DMC1 Antibody (1D12/4)
    Name: Sumit sandhu
    Application: Immunofluorescence
    Sample Tested: Mouse spermatocytes
    Species: Mouse
    Verified Customer | Posted 06/29/2020
    DMC1 Antibody (1D12/4) - BSA Free NB100-181
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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Protocols

View specific protocols for DMC1 Antibody (1D12/4) - BSA Free (NB100-181):

DMC1 Antibody (1D12/4):
Western Blot
Procedure Guide for NB 100-181 Anti-hDMC-1

Western Blot
1. Proteins are separated on an SDS-PAGE gel.

2. Following the protein transfer, the membrane is blocked with 5% NFDM in TBS-T.

3. The membrane is then washed.

4. NB 100-181 [anti-hDMC] is diluted 1:1,000 in dilution buffer [5% NFDM in TBS-T] and incubated for 3 hours at room temperature, gently shaking.

5. The membrane is then washed.

6. Secondary antibody [HRP conjugated goat anti-mouse] is incubated for 45 minutes at room temperature, gently shaking.

7. Develop membrane.

NOTE: Mouse testis tissue extracts were used as a positive control for this antibody. MEF 161 and HeLa whole cell extracts were not shown to work in Western analysis.

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FAQs

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