Catalog # Availability Size / Price Qty
DMHBO+ | CAS No. 2322286-81-5 | Light-up Aptamers
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Description: Fluorescent upon binding to Chili aptamer; for imaging RNA in cells

Chemical Name: 4-(4-((Z)-4-Hydroxy-3,5-dimethoxybenzylidene)-2-((hydroxyimino)methyl)-5-oxo-4,5-dihydro-1H-imidazol-1-yl)-N,N,N-trimethylbenzenaminium iodide

Purity: ≥98%

Product Details

Biological Activity

DMHBO+ is a cationic fluorophore. Its fluorescence is activated by binding to chili aptamer (Kd = 12 nM). Chili-DMHBO+ complex mimics red fluorescent proteins, suitable for imaging RNA in cells. It is an ideal FRET donor to the rhodamine dye Atto 590 and has applications in FRET-based analytical RNA systems. Excitation/emission maxima (λ) = 456/592 nm; quantum yield = 0.1; stokes shift = 136 nm.

Optical Data for DMHBO+

λabs 456 nm
λem 592 nm
Quantum Yield (φ) 0.1
Application Time-resolved fluorescence energy transfer (TR-FRET)

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Technical Data

Soluble to 50 mM in DMSO
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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