DNA-PKcs Antibody

Novus Biologicals | Catalog # NB100-657

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all DNA-PKcs Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1850 and 1900 of human DNA-Dependent Protein Kinase, catalytic subunit using the numbering given in entry NP_008835.5 (GeneID 5591).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DNA-PKcs Antibody

Western Blot: DNA-PKcs Antibody [NB100-657]

Western Blot: DNA-PKcs Antibody [NB100-657]

Western Blot: DNA-PKcs Antibody [NB100-657] - MDA-MB-231, MCF-7, SUM149, SUM159, T47D, HCC1954, whole cell lysates (50 ug/lane). 10% SDS-PAGE. DNA-PKcs (NB100-657) primary antibody at 1:1000, 4C, overnight. Western blot image submitted by a verified customer review.
Western Blot: DNA-PKcs Antibody [NB100-657]

Western Blot: DNA-PKcs Antibody [NB100-657]

Western Blot: DNA-PKcs Antibody [NB100-657] - Detection of Human DNA-PKcs on HeLa whole cell lysate using NB100-657. DNA-PKcs antibodies NB100-658, NB100-659 and NB100-660.

Applications for DNA-PKcs Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 5 ug/mg lysate

Western Blot

1:1000 - 1:10000
Application Notes
This antibody is useful for Western Blot and Immunoprecipitation (Ku80 immunoprecipitates along with DNA-PKcs when using this antibody). Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. In some cases, the antibody may be diluted further than indicated. DNA-PKcs antibody validated for WB from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NB100-657 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS, 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DNA-PKcs

DNA PK is the key component of the non-homologous end-joining (NHEJ) pathway of DSB repair in mammalian cells. DNA PK consists of a heterodimeric DNA-binding subunit (Ku70/80) and an approximately 465 kDa catalytic subunit (DNA PKcs). DNA PKcs is a serine/threonine protein kinase whose activity is greatly stimulated by its recruitment to DNA breaks by the Ku heterodimer.

Long Name

DNA-dependent Protein Kinase Catalytic Subunit

Alternate Names

DNAPK, DNAPKcs, DNPK1, HYRC1, p350, PRKDC, XRCC7

Entrez Gene IDs

5591 (Human)

Gene Symbol

PRKDC

UniProt

NP_008835.5 (Human)

Additional DNA-PKcs Products

Product Documents for DNA-PKcs Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DNA-PKcs Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

DNA Repair

Citations for DNA-PKcs Antibody

Customer Reviews for DNA-PKcs Antibody (1)

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  • DNA-PKcs Antibody
    Name: Yongkang Yang
    Application: Western Blot
    Sample Tested: cell line, whole cell lysate
    Species: Human
    Verified Customer | Posted 10/10/2020
    Western Blot: MDA-MB-231. MCF-7, SUM149, SUM159, T47D, HCC1954, whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. DNA-PKcs (NB100-657) primary antibody: 1:1000, 4℃, overnight.
    DNA-PKcs Antibody NB100-657

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