DOT1L Antibody (7A10H9)

Novus Biologicals | Catalog # NBP3-15446

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7A10H9 expressed in HEK293
View all DOT1L Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DOT1L (NP_115871.1). MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNT

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DOT1L Antibody (7A10H9)

Western Blot: DOT1L Antibody (7A10H9) [NBP3-15446]

Western Blot: DOT1L Antibody (7A10H9) [NBP3-15446]

Western Blot: DOT1L Antibody (7A10H9) [NBP3-15446] - Western blot analysis of extracts of various cell lines, using DOT1L Rabbit mAb (NBP3-15446) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunoprecipitation: DOT1L Antibody (7A10H9) [NBP3-15446]

Immunoprecipitation: DOT1L Antibody (7A10H9) [NBP3-15446]

Immunoprecipitation: DOT1L Antibody (7A10H9) [NBP3-15446] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug DOT1L antibody (NBP3-15446). Western blot was performed from the immunoprecipitate using DOT1L antibody (NBP3-15446) at a dilition of 1:1000.

Applications for DOT1L Antibody (7A10H9)

Application
Recommended Usage

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DOT1L

DOT1 is an evolutionarily conserved histone methyltransferase that methylates lysine-79 of histone H3 in the core domain. Unlike other histone methyltransferases, DOT1 does not contain a SET domain, and it specifically methylates nucleosomal histone H3.

Long Name

Disruptor of Telomeric Silencing 1-like

Alternate Names

H3-K79-HMTase, KMT4

Gene Symbol

DOT1L

Additional DOT1L Products

Product Documents for DOT1L Antibody (7A10H9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for DOT1L Antibody (7A10H9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

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