Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all DPPA4 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human DPPA4.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for DPPA4 Antibody - Azide and BSA Free

Western Blot: DPPA4 Antibody [NBP2-98786]

Western Blot: DPPA4 Antibody [NBP2-98786]

Western Blot: DPPA4 Antibody [NBP2-98786] - Anti-DPPA4 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 38 kDa (We are unsure as to the identity of these extra bands).
Immunoprecipitation: DPPA4 Antibody [NBP2-98786]

Immunoprecipitation: DPPA4 Antibody [NBP2-98786]

Immunoprecipitation: DPPA4 Antibody [NBP2-98786] - DPPA4 was immunoprecipitated using: Lane A: 0.5 mg A549 Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate. Lane C: 0.5 mg Jurkat Whole Cell Lysate4 ul anti-DPPA4 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-DPPA4 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 40 kDa

Applications for DPPA4 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunoprecipitation

4-8 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DPPA4

DPPA4, also known as Developmental pluripotency-associated protein 4, is a 33.5 kDa, 304 amino acid protein that is involved in inhibition of embryonic cells differentiation and maintenance of target genes' active epigenetic status. Current research is being conducted on diseases such as embryonal and other forms of carcinoma. The protein interacts with RPS6KB1, VIM, DPPA2, PTPRO, and SH3GL3. Pathways of the protein are unknown.

Long Name

Developmental Pluripotency Associated 4

Alternate Names

developmental pluripotency associated 4, developmental pluripotency-associated protein 4, FLJ10713,2410091M23Rik

Gene Symbol

DPPA4

Additional DPPA4 Products

Product Documents for DPPA4 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for DPPA4 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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