Human DPPIV/CD26 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP1-91180

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum (20 uL)

Sensitivity

7.3 ng/mL

Assay Range

15.6 - 500 ng/mL
View all DPPIV/CD26 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human DPPIV/CD26 positive serum. No crossreactivity was detected.

Description

The human sCD26 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sCD26

Precision

Intra-Assay Precision (Precision within an assay) CV% 4.60%

Inter-Assay Precision (Precision between assays) CV% 9.10%

Recovery for Human DPPIV/CD26 ELISA Kit (Colorimetric)

Recovery

95%

Scientific Data Images for Human DPPIV/CD26 ELISA Kit (Colorimetric)

ELISA: Human DPPIV/CD26 ELISA Kit (Colorimetric) [NBP1-91180]

ELISA: Human DPPIV/CD26 ELISA Kit (Colorimetric) [NBP1-91180]

ELISA: DPPIV/CD26 ELISA Kit [NBP1-91180] - Standard curve

Kit Contents for Human DPPIV/CD26 ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sCD26
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-human sCD26 monoclonal antibody
  • Human sCD26 Standard, 500 ng/ml upon dilution
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: DPPIV/CD26

D26, a T cell activation antigen, is a 110kDa cell surface glycoprotein with known dipeptidyl peptidase IV activity in its extracellular domain which is present on various cell types, including T cells and epithelial cells of the liver, kidney, and intestine. Of the T cell antigens described to date, CD26 has proved to be one of the most intriguing in the scope of its functional associations. Considerable evidence suggests that CD26 can deliver a potent costimulatory signal to T-cells. This signal transducing property appears to be a property of its extracellular domain. In addition, CD26 appears to be a functional collagen receptor that may aid activated T-cells in localizing to inflammatory regions. It has also been demonstrated that CD26 not only acts as a functional dipeptidyl peptidase IV, but also binds strongly to adenosine deaminase. Significant levels of DPPIV activity have been shown to exist in plasma, serum, and urine. The serum form of DPPIV is unique, and is not a breakdown product of membrane CD26, nevertheless exposing significant structural similarity to the membrane form.

Long Name

Dipeptidyl-peptidase IV

Alternate Names

CD26, DPP4

Gene Symbol

DPP4

Additional DPPIV/CD26 Products

Product Documents for Human DPPIV/CD26 ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Human DPPIV/CD26 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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