dTAG-Fluorescein

Tocris Bioscience | Catalog # 7892

Fluorescent probe for labeling mutant FKBP12 (F36V) proteins
Tocris Bioscience

Key Product Details

Description

Fluorescent probe for labeling mutant FKBP12 (F36V) proteins

Wavelength

Excitation = 493 nm, Emission = 514 nm
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Product Description

dTAG-Fluorescein is a fluorescent probe for labeling FKBP12F36V fusion proteins. Comprises a high-affinity ligand for FKBP12F36V mutant protein (Kd in the subnanomolar range and 1,000-fold selectivity for the F36V mutant of FKBP12 over wild-type) coupled to 5-amino fluorescein. dTAG-Fluorescein can fluorescently label FKBP12F36V fusion proteins expressed at different levels in a variety of cell lines.

Optical Data

dTAG-Fluorescein Dye Spectra
Emission Color Green
λabs 493 nm
λem 514 nm
Extinction Coefficient (ε) 68800 M-1cm-1
Closest Laser line 488 nm
Application Confocal microscopy

Product Specifications for dTAG-Fluorescein

Molecular Weight

1023.10

Formula

C58H58N2O15

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

(R)-1-(2-(2-((3',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthen]-5-yl)amino)-2-oxoethoxy)phenyl)-3-(3,4-dimethoxyphenyl)propyl (S)-1-((S)-2-(3,4,5-trimethoxyphenyl)butanoyl)piperidine-2-carboxylate

InChI Key

ZSRZNOZTAUPBBF-ZSDGZGBPSA-N

SMILES

CC[C@@H](C1=CC(OC)=C(C(OC)=C1)OC)C(N2CCCC[C@H]2C(O[C@@H](C3=C(C=CC=C3)OCC(NC4=CC(C(O5)=O)=C(C=C4)C65C7=C(C=C(C=C7)O)OC8=C6C=CC(O)=C8)=O)CCC9=CC(OC)=C(C=C9)OC)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 10.23 10

Preparing Stock Solutions for dTAG-Fluorescein

The following data is based on the product molecular weight 1023.10.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.1 mM 9.77 mL 48.87 mL 97.74 mL
0.5 mM 1.95 mL 9.77 mL 19.55 mL
1 mM 0.98 mL 4.89 mL 9.77 mL
5 mM 0.20 mL 0.98 mL 1.95 mL

Calculators

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Background References

References are publications that support the biological activity of the product.

Product Documents for dTAG-Fluorescein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for dTAG-Fluorescein

For research use only

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