Dtk/TYRO3 Antibody (JE38-13)

Novus Biologicals | Catalog # NBP3-33070

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE38-13 expressed in HEK293
View all Tyro3/Dtk Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human Dtk/TYRO3 aa 841-890 / 890. (Uniprot: Q06418)

Localization

Cell membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Dtk/TYRO3 Antibody (JE38-13)

Dtk/TYRO3 Antibody (JE38-13)

Western Blot: Dtk/TYRO3 Antibody (JE38-13) [NBP3-33070]

Western Blot: Dtk/TYRO3 Antibody (JE38-13) [NBP3-33070] - Western blot analysis of Dtk/TYRO3 on different lysates with Rabbit anti-Dtk/TYRO3 antibody (NBP3-33070) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (20 ug/Lane)
Lane 2: HCT 116 cell lysate (20 ug/Lane)
Lane 3: MCF7 cell lysate (20 ug/Lane)
Lane 4: SH-SY5Y cell lysate (20 ug/Lane)
Lane 5: PC-12 cell lysate (20 ug/Lane)
Lane 6: Human brain tissue lysate (40 ug/Lane)

Predicted band size: 92 kDa
Observed band size: 92 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33070) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Applications for Dtk/TYRO3 Antibody (JE38-13)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tyro3/Dtk

Receptor protein tyrosine kinases (RPTKs) are a class of transmembrane signaling molecules. They have been shown to regulate signaling cascades that control synaptic function in the central nervous system (CNS). Tyro3 is one of three RPTKs belonging to the Axl/UFO subfamily, that also includes Axl and Mer. Tyro3 is the most commonly expressed of these receptors in the CNS. It may be involved in the cell adhesion process, and is commonly found in adult tissues with expression mostly in brain, testis, vascular and lymphatic tissue.

Long Name

Tyrosine Protein Kinase Receptor TYRO3

Alternate Names

Brt, Dtk, Rse, Sky

Gene Symbol

TYRO3

Additional Tyro3/Dtk Products

Product Documents for Dtk/TYRO3 Antibody (JE38-13)

Certificate of Analysis

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Product Specific Notices for Dtk/TYRO3 Antibody (JE38-13)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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