Recombinant Human DUSP10 His Protein

Novus Biologicals | Catalog # NBP1-78891

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

Enzyme Activity, SDS-PAGE
View all DUSP10 Proteins and Enzymes »

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 149-482 of Human DUSP10

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMIIYPN DLAKKMTKCS KSHLPSQGPV IIDCRPFMEY NKSHIQGAVH INCADKISRR RLQQGKITVL DLISCREGKD SFKRIFSKEI IVYDENTNEP SRVMPSQPLH IVLESLKREG KEPLVLKGGL SSFKQNHENL CDNSLQLQEC REVGGGASAA SSLLPQPIPT TPDIENAELT PILPFLFLGN EQDAQDLDTM QRLNIGYVIN VTTHLPLYHY EKGLFNYKRL PATDSNKQNL RQYFEEAFEF IEEAHQCGKG LLIHCQAGVS RSATIVIAYL MKHTRMTMTD AYKFVKGKRP IISPNLNFMG QLLEFEEDLN NGVTPRILTP KLMGVETVV

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

40.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 600 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human DUSP10 His Protein

Recombinant Human DUSP10 Protein [NBP1-78891]

Formulation, Preparation, and Storage

NBP1-78891
Formulation 20 mM Tris-HCl buffer (pH 8.0), 2 mM DTT, 50% glycerol, 200 mM NaCl
Preservative No Preservative
Concentration 0.25 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DUSP10

DUSP10 (Dual specificity protein phosphatase 10) belongs to the protein-tyrosine phosphatase family. Dual specificity protein phosphatases(DUSPs) inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAPK superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. DUSP10 has been shown to interact with MAPK14 and MAPK8. When expressed in mammalian cells, DUSP10 blocks the enzymatic activation of MAP kinases with the selectivity p38 approximately JNK/SAPK >> ERK. Recombinant human DUSP10 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

dual specificity phosphatase 10, dual specificity phosphatase MKP-5, EC 3.1.3.16, EC 3.1.3.48, MAP kinase phosphatase 5, Mitogen-activated protein kinase phosphatase 5, MKP-5dual specificity protein phosphatase 10, MKP5serine/threonine specific protein phosphatase

Gene Symbol

DUSP10

Additional DUSP10 Products

Product Documents for Recombinant Human DUSP10 His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human DUSP10 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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