DUSP8 Antibody (HL2338)

Novus Biologicals | Catalog # NBP3-48677

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2338
View all DUSP8 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human DUSP8

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DUSP8 Antibody (HL2338)

DUSP8 Antibody (HL2338)

DUSP8 Antibody (HL2338)-NBP3-48677

MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DUSP8 stained by DUSP8 antibody [HL2338] diluted at 1:500.Blue: Fluoroshield with DAPI.
DUSP8 Antibody (HL2338)

DUSP8 Antibody (HL2338)-NBP3-48677

Human tissue extract (5 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DUSP8 antibody [HL2338] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301 was used to detect the primary antibody.
DUSP8 Antibody (HL2338)

Western Blot: DUSP8 Antibody (HL2338) [NBP3-48677] -

Non-transfected (-) and transfected (+) MCF-7 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DUSP8 antibody [HL2338] (NBP3-48677) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
DUSP8 Antibody (HL2338)

Western Blot: DUSP8 Antibody (HL2338) [NBP3-48677] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DUSP8 antibody [HL2338] (NBP3-48677) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
DUSP8 Antibody (HL2338)

Western Blot: DUSP8 Antibody (HL2338) [NBP3-48677] -

Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DUSP8 antibody [HL2338] (NBP3-48677) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data are based on NCBI database.

Applications for DUSP8 Antibody (HL2338)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DUSP8

DUSP8 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP8 inactivates SAPK/JNK and p38, is expressed predominantly in the adult brain, heart, and skeletal muscle, is localized in the cytoplasm, and is induced by nerve growth factor and insulin. An intronless pseudogene for DUSP8 is present on chromosome 10q11.2.

Alternate Names

C11orf81, chromosome 11 open reading frame 81, dual specificity phosphatase 8, Dual specificity protein phosphatase hVH-5, EC 3.1.3.16, EC 3.1.3.48, FLJ42476, FLJ42958, HVH8, serine/threonine specific protein phosphatase, vaccinia virus homolog, VH5

Gene Symbol

DUSP8

Additional DUSP8 Products

Product Documents for DUSP8 Antibody (HL2338)

Certificate of Analysis

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Product Specific Notices for DUSP8 Antibody (HL2338)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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