dUTPase Antibody (HL1210) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25445

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1210 expressed in HEK293

Format

Azide and BSA Free
View all dUTPase Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human dUTPase. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (86%), Bovine (89%), Chicken (82%).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for dUTPase Antibody (HL1210) - Azide and BSA Free

dUTPase Antibody (HL1210) - Azide and BSA Free

Immunohistochemistry-Paraffin: dUTPase Antibody (HL1210) - Azide and BSA Free [NBP3-25445] -

Immunohistochemistry-Paraffin: dUTPase Antibody (HL1210) - Azide and BSA Free [NBP3-25445] - DUT antibody [HL1210] detects DUT protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human A549. DUT stained by DUT antibody [HL1210] (NBP3-25445) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
dUTPase Antibody (HL1210) - Azide and BSA Free

Western Blot: dUTPase Antibody (HL1210) - Azide and BSA Free [NBP3-25445] -

Western Blot: dUTPase Antibody (HL1210) - Azide and BSA Free [NBP3-25445] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with DUT antibody [HL1210] (NBP3-25445) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
dUTPase Antibody (HL1210) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: dUTPase Antibody (HL1210) - Azide and BSA Free [NBP3-25445] -

Immunocytochemistry/Immunofluorescence: dUTPase Antibody (HL1210) - Azide and BSA Free [NBP3-25445] - DUT antibody [HL1210] detects DUT protein at cytoplasm, nucleus and centrosome by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DUT stained by DUT antibody [HL1210] (NBP3-25445) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.

Applications for dUTPase Antibody (HL1210) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: dUTPase

dUTPase encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.

Alternate Names

deoxyuridine triphosphatase, dUTP pyrophosphatasedUTP nucleotidohydrolase, dUTPasedeoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial, EC 3.6.1.23, FLJ20622

Gene Symbol

DUT

Additional dUTPase Products

Product Documents for dUTPase Antibody (HL1210) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for dUTPase Antibody (HL1210) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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