DYKDDDDK Epitope Tag Antibody - BSA Free

Novus Biologicals | Catalog # NB600-344

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Epitope Tag

Cited:

Primate

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free
View all DYKDDDDK Epitope Tag Primary Antibodies »

Product Specifications

Immunogen

To produce DYKDDDDK Epitope Tag Antibody, Goats were immunized with ECS, Enterokinase Cleavage Site (xxxDDDDK), conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.

Specificity

DYKDDDDK Epitope Tag Antibody reacts with FLAG.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

1.01 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DYKDDDDK Epitope Tag Antibody - BSA Free

Western Blot: DYKDDDDK Epitope Tag AntibodyBSA Free [NB600-344]

Western Blot: DYKDDDDK Epitope Tag AntibodyBSA Free [NB600-344]

Western Blot: DYKDDDDK Epitope Tag Antibody [NB600-344] - Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody: Affinity purified goat DYKDDDDK Epitope Tag Antibody [NB600-344] used for WB at 0.04 ug/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds. Observed molecular weight ~49 kDa.
Western Blot: DYKDDDDK Epitope Tag AntibodyBSA Free [NB600-344]

Western Blot: DYKDDDDK Epitope Tag AntibodyBSA Free [NB600-344]

Western Blot: DYKDDDDK Epitope Tag Antibody [NB600-344] - Analysis using the Agarose Immobilized conjugate of DYKDDDDK Epitope Tag Antibody [NB600-344]. DYKDDDK in transfected COS-7 cells using agarose immobilized DYKDDDDK Epitope Tag Antibody.
Immunocytochemistry/ Immunofluorescence: DYKDDDDK Epitope Tag Antibody - BSA Free [NB600-344]

Immunocytochemistry/ Immunofluorescence: DYKDDDDK Epitope Tag Antibody - BSA Free [NB600-344]

DYKDDDDK-Epitope-Tag-Antibody-Immunocytochemistry-Immunofluorescence-NB600-344-img0013.jpg
Immunocytochemistry/ Immunofluorescence: DYKDDDDK Epitope Tag Antibody - BSA Free [NB600-344]

Immunocytochemistry/ Immunofluorescence: DYKDDDDK Epitope Tag Antibody - BSA Free [NB600-344]

Immunocytochemistry/Immunofluorescence: DYKDDDDK Epitope Tag Antibody [NB600-344] - Samples: Human microvascular endothelial cells expressing FLAG tagged beta-catenin following transient transfection. Antibody: Affinity purified goat DYKDDDDK Epitope Tag Antibody [NB600-344] used at 1 ug/ml. Detection: FITC labeled rabbit anti-goat IgG (h+l).
DYKDDDDK Epitope Tag Antibody - BSA Free

Proximity Ligation Assay: DYKDDDDK Epitope Tag Antibody - BSA Free [NB600-344] -

Expression of the ZFN as detected by PLA.PK-15 cells were transfected with the pZFN1 or pZFN2 vectors expressing the 3xFLAG tagged fluorescent proteins ZFN1-CFP or ZFN2-YFP, the nucleus was stained by DAPI and a PLA against the FLAG epitope was performed demonstrating the specific localization of the ZFN in the nucleus. Scale bars, 5 μm.
DYKDDDDK Epitope Tag Antibody - BSA Free

Proximity Ligation Assay: DYKDDDDK Epitope Tag Antibody - BSA Free [NB600-344] -

Proximity Ligation Assay: DYKDDDDK Epitope Tag Antibody - BSA Free [NB600-344] - Expression of the ZFN as detected by PLA.PK-15 cells were transfected with the pZFN1 or pZFN2 vectors expressing the 3xFLAG tagged fluorescent proteins ZFN1-CFP or ZFN2-YFP, the nucleus was stained by DAPI & a PLA against the FLAG epitope was performed demonstrating the specific localization of the ZFN in the nucleus. Scale bars, 5 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25909512), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
DYKDDDDK Epitope Tag Antibody - BSA Free

Western Blot: DYKDDDDK Epitope Tag Antibody - BSA Free [NB600-344] -

SCAMP5 facilitates the secretion of alpha -synuclein through exosome pathway.(A) Flowchart showing the exosome extraction procedure. (B) Quality control of exosome extraction. Cell lysates(CL), Cell medium(CM), exosome(EXO), Flow through(FT), and RIPA insoluable pellets of EGFP-alpha -synuclein stable overexpressed SH-SY5Y cells were collected according to the flowchart in 6A. The difference in apparent molecular weight was mainly due to different buffer between samples. Immunoblotting of exosome marker CD63 is conducted using native lysis buffer and native PAGE. Other proteins such as alpha -synuclein and ER marker Calnexin were examined with SDS-PAGE. (C) Electron microscopy images of exosomes isolated from the cell medium of SH-SY5Y cells stably expressing EGFP-alpha -synuclein. (D) SCAMP5 is abundant in exosomes, and it increases alpha -synuclein secretion via exosome. SH-SY5Y cells stably expressing EGFP-alpha -synuclein were transfected with Flag-GFP or Flag-SCAMP5, and harvested 48 hour later. All the samples were processed identically. The loading/total volumes of CL, CM, EXO, and FT were 2/1,200μL, 30/6,000μL, 30/200μL, 30/6,000μL respectively. (E) Quantification of secreted EGFP-alpha -synuclein in exosome of SH-SY5Y cells overexpressed with SCAMP5 or control in three independent experiments. (mean +/-S.E.M.; n = 3; *p<0.05). (F) SH-SY5Y cells stably expressing EGFP-alpha -synuclein were transfected with Flag-GFP or Flag-SCAMP5, and harvested 48 hour later. BrefeldinA was added 6 hours before harvest with a change of medium. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28700687), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
DYKDDDDK Epitope Tag Antibody - BSA Free

Western Blot: DYKDDDDK Epitope Tag Antibody - BSA Free [NB600-344] -

SCAMP5 facilitates the secretion of alpha -synuclein through exosome pathway.(A) Flowchart showing the exosome extraction procedure. (B) Quality control of exosome extraction. Cell lysates(CL), Cell medium(CM), exosome(EXO), Flow through(FT), and RIPA insoluable pellets of EGFP-alpha -synuclein stable overexpressed SH-SY5Y cells were collected according to the flowchart in 6A. The difference in apparent molecular weight was mainly due to different buffer between samples. Immunoblotting of exosome marker CD63 is conducted using native lysis buffer and native PAGE. Other proteins such as alpha -synuclein and ER marker Calnexin were examined with SDS-PAGE. (C) Electron microscopy images of exosomes isolated from the cell medium of SH-SY5Y cells stably expressing EGFP-alpha -synuclein. (D) SCAMP5 is abundant in exosomes, and it increases alpha -synuclein secretion via exosome. SH-SY5Y cells stably expressing EGFP-alpha -synuclein were transfected with Flag-GFP or Flag-SCAMP5, and harvested 48 hour later. All the samples were processed identically. The loading/total volumes of CL, CM, EXO, and FT were 2/1,200μL, 30/6,000μL, 30/200μL, 30/6,000μL respectively. (E) Quantification of secreted EGFP-alpha -synuclein in exosome of SH-SY5Y cells overexpressed with SCAMP5 or control in three independent experiments. (mean +/-S.E.M.; n = 3; *p<0.05). (F) SH-SY5Y cells stably expressing EGFP-alpha -synuclein were transfected with Flag-GFP or Flag-SCAMP5, and harvested 48 hour later. BrefeldinA was added 6 hours before harvest with a change of medium. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28700687), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for DYKDDDDK Epitope Tag Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:500 (coating); 1:1000-1:30000 (primary)

Immunocytochemistry/ Immunofluorescence

1:100-1:400

Immunoprecipitation

1-4 ug/mg Lysate

Western Blot

1:1000-1:30000
Application Notes
Use in IP, WB, and ICC/IF reported in multiple pieces of scientific literature.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DYKDDDDK Epitope Tag

Fusion proteins, in which a known peptide such as a His-Tag, GST-Tag, or DYKDDDDK (FLAG(R)) is cloned with the target of interest, were developed to simplify protein isolation. Since specific antibodies may not be available against the protein of interest, epitope tag antibodies are commonly used to detect protein expression.

The epitope tag, DYKDDDDK, is comprised of an eight amino acid sequence derived from the gene-10 product from bacteriophage T7 and contains an enterokinase-cleavage site (1). Its hydrophilic nature and short peptide sequence reduce possible interference with protein expression, post-translational modifications, protein interactions, and protein function (2). The DYKDDDDK (FLAG(R)) epitope tag can either be fused to the N-terminus, C-terminus, or placed at an internal position of the target protein, facilitating characterization of the protein of interest using immunodetection methods. Compared to the standard DYKDDDDK epitope tag antibody, the rat monoclonal DYKDDDDK Epitope Tag antibody (clone L5) is 2-8 times more sensitive (3).

References

1. Brizzard BL, Chubet RG, Vizard DL. (1994) Immunoaffinity purification of (FLAG(R)) epitope-tagged bacterial alkaline phosphatase using a novel monoclonal antibody and peptide elution. Biotechniques. 16(4):730-5. PMID: 8024796

2. Einhauer A, Jungbauer A. (2001) The (FLAG(R)) peptide, a versatile fusion tag for the purification of recombinant proteins. J Biochem Biophys Methods. 49(1-3):455-65. PMID: 11694294

3. Park SH, Cheong C, Idoyaga J, Kim JY, Choi JH, Do Y, Lee H, Jo JH, Oh YS, Im W, Steinman RM, Park CG. (2008) Generation and application of new rat monoclonal antibodies against synthetic DYKDDDDK Epitope Tag (FLAG(R)) and OLLAS tags for improved immunodetection. J Immunol Methods. 331(1-2):27-38. PMID: 18054954

FLAG(R) is a trademark of Sigma-Aldrich.

Alternate Names

FLAG Tag

Additional DYKDDDDK Epitope Tag Products

Product Documents for DYKDDDDK Epitope Tag Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for DYKDDDDK Epitope Tag Antibody - BSA Free

FLAG(R) and ANTI-FLAG(R) are registered trademarks of Sigma-Aldrich Co. LLC.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DYKDDDDK Epitope Tag Antibody - BSA Free

Customer Reviews for DYKDDDDK Epitope Tag Antibody - BSA Free

There are currently no reviews for this product. Be the first to review DYKDDDDK Epitope Tag Antibody - BSA Free and earn rewards!

Have you used DYKDDDDK Epitope Tag Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for DYKDDDDK Epitope Tag Antibody - BSA Free

Showing  1 - 5 of 7 FAQs Showing All

  • Q: Are the FLAG Tag antibodies validated in Simple Western?

    A: Yes, we offer 3 FLAG Tag antibodies that have been tested in Simple Western: NBP1-71705, NBP1-97410, and MAB8529.

  • Q: Do FLAG Tag antibodies comes in lyophilized form?

    A: The following FLAG Tag antibodies come in lyophilized form: NBP1-97393, NBP1-97401, NBP1-97399, NBP1-97400, and NBP1-97402.

  • Q: Is this antibody suitable for ChIP analysis of an N-terminally tagged protein?

    A: Our DYKDDDDK epitope tag antibody with catalogue number NB600-344 has been validated for Western blotting, immunofluorescence, immunoprecipitation and ELISA but has not yet been tested for ChIP. However, our DYKDDDDK Epitope Tag antibody with catalog number NBP2-59146 is validated for use in ChIP.

  • Q: What is the immunogen sequence of this FLAG Tag antibody? 

    A: This antibody was made to a ECS enterokinase cleavage site (xxxDDDDK) conjugated to KLH.  

  • Q: What the theoretical molecular weight for FLAG Tag antibodies?

    A: The TMW for DYKDDDDK epitope FLAG Tag is approximately 1.01 kDa.

  • Q: Will FLAG Tag antibodies detect internal FLAG tags? My FLAG sequence is sandwiched between two other protein sequences.

    A: If you are applying your sample(s) to SDS-PAGE, under reducing condition and follow by Western Blot, DYKDDDDK epitope tag antibodies should be able to detect the tag region located internally. However, it depends on the property of your chimeric protein and if your tag is in the hydrophobic or hydrophilic region. Although DYKDDDDK is very hydrophilic, it may not be exposed to the surface if embedded deeply inside the protein with a high content of hydrophobic amino acids with or without covalently-linked modifications such as lipid moieties.

  • Q: Will FLAG Tag antibodies recognize FLAG at the C-terminal end?

    A: Yes, FLAG Tag antibodies can recognize FLAG-tag at both the N- or C-terminal.

  • Q: Are the FLAG Tag antibodies validated in Simple Western?

    A: Yes, we offer 3 FLAG Tag antibodies that have been tested in Simple Western: NBP1-71705, NBP1-97410, and MAB8529.

  • Q: Do FLAG Tag antibodies comes in lyophilized form?

    A: The following FLAG Tag antibodies come in lyophilized form: NBP1-97393, NBP1-97401, NBP1-97399, NBP1-97400, and NBP1-97402.

  • Q: Is this antibody suitable for ChIP analysis of an N-terminally tagged protein?

    A: Our DYKDDDDK epitope tag antibody with catalogue number NB600-344 has been validated for Western blotting, immunofluorescence, immunoprecipitation and ELISA but has not yet been tested for ChIP. However, our DYKDDDDK Epitope Tag antibody with catalog number NBP2-59146 is validated for use in ChIP.

  • Q: What is the immunogen sequence of this FLAG Tag antibody? 

    A: This antibody was made to a ECS enterokinase cleavage site (xxxDDDDK) conjugated to KLH.  

  • Q: What the theoretical molecular weight for FLAG Tag antibodies?

    A: The TMW for DYKDDDDK epitope FLAG Tag is approximately 1.01 kDa.

  • Q: Will FLAG Tag antibodies detect internal FLAG tags? My FLAG sequence is sandwiched between two other protein sequences.

    A: If you are applying your sample(s) to SDS-PAGE, under reducing condition and follow by Western Blot, DYKDDDDK epitope tag antibodies should be able to detect the tag region located internally. However, it depends on the property of your chimeric protein and if your tag is in the hydrophobic or hydrophilic region. Although DYKDDDDK is very hydrophilic, it may not be exposed to the surface if embedded deeply inside the protein with a high content of hydrophobic amino acids with or without covalently-linked modifications such as lipid moieties.

  • Q: Will FLAG Tag antibodies recognize FLAG at the C-terminal end?

    A: Yes, FLAG Tag antibodies can recognize FLAG-tag at both the N- or C-terminal.

  • Q: Are the FLAG Tag antibodies validated in Simple Western?

    A: Yes, we offer 3 FLAG Tag antibodies that have been tested in Simple Western: NBP1-71705, NBP1-97410, and MAB8529.

  • Q: Do FLAG Tag antibodies comes in lyophilized form?

    A: The following FLAG Tag antibodies come in lyophilized form: NBP1-97393, NBP1-97401, NBP1-97399, NBP1-97400, and NBP1-97402.

  • Q: Is this antibody suitable for ChIP analysis of an N-terminally tagged protein?

    A: Our DYKDDDDK epitope tag antibody with catalogue number NB600-344 has been validated for Western blotting, immunofluorescence, immunoprecipitation and ELISA but has not yet been tested for ChIP. However, our DYKDDDDK Epitope Tag antibody with catalog number NBP2-59146 is validated for use in ChIP.

  • Q: What is the immunogen sequence of this FLAG Tag antibody? 

    A: This antibody was made to a ECS enterokinase cleavage site (xxxDDDDK) conjugated to KLH.  

  • Q: What the theoretical molecular weight for FLAG Tag antibodies?

    A: The TMW for DYKDDDDK epitope FLAG Tag is approximately 1.01 kDa.

  • Q: Will FLAG Tag antibodies detect internal FLAG tags? My FLAG sequence is sandwiched between two other protein sequences.

    A: If you are applying your sample(s) to SDS-PAGE, under reducing condition and follow by Western Blot, DYKDDDDK epitope tag antibodies should be able to detect the tag region located internally. However, it depends on the property of your chimeric protein and if your tag is in the hydrophobic or hydrophilic region. Although DYKDDDDK is very hydrophilic, it may not be exposed to the surface if embedded deeply inside the protein with a high content of hydrophobic amino acids with or without covalently-linked modifications such as lipid moieties.

  • Q: Will FLAG Tag antibodies recognize FLAG at the C-terminal end?

    A: Yes, FLAG Tag antibodies can recognize FLAG-tag at both the N- or C-terminal.

  • Q: Are the FLAG Tag antibodies validated in Simple Western?

    A: Yes, we offer 3 FLAG Tag antibodies that have been tested in Simple Western: NBP1-71705, NBP1-97410, and MAB8529.

  • Q: Do FLAG Tag antibodies comes in lyophilized form?

    A: The following FLAG Tag antibodies come in lyophilized form: NBP1-97393, NBP1-97401, NBP1-97399, NBP1-97400, and NBP1-97402.

  • Q: Is this antibody suitable for ChIP analysis of an N-terminally tagged protein?

    A: Our DYKDDDDK epitope tag antibody with catalogue number NB600-344 has been validated for Western blotting, immunofluorescence, immunoprecipitation and ELISA but has not yet been tested for ChIP. However, our DYKDDDDK Epitope Tag antibody with catalog number NBP2-59146 is validated for use in ChIP.

  • Q: What is the immunogen sequence of this FLAG Tag antibody? 

    A: This antibody was made to a ECS enterokinase cleavage site (xxxDDDDK) conjugated to KLH.  

  • Q: What the theoretical molecular weight for FLAG Tag antibodies?

    A: The TMW for DYKDDDDK epitope FLAG Tag is approximately 1.01 kDa.

  • Q: Will FLAG Tag antibodies detect internal FLAG tags? My FLAG sequence is sandwiched between two other protein sequences.

    A: If you are applying your sample(s) to SDS-PAGE, under reducing condition and follow by Western Blot, DYKDDDDK epitope tag antibodies should be able to detect the tag region located internally. However, it depends on the property of your chimeric protein and if your tag is in the hydrophobic or hydrophilic region. Although DYKDDDDK is very hydrophilic, it may not be exposed to the surface if embedded deeply inside the protein with a high content of hydrophobic amino acids with or without covalently-linked modifications such as lipid moieties.

  • Q: Will FLAG Tag antibodies recognize FLAG at the C-terminal end?

    A: Yes, FLAG Tag antibodies can recognize FLAG-tag at both the N- or C-terminal.

  • Q: Are the FLAG Tag antibodies validated in Simple Western?

    A: Yes, we offer 3 FLAG Tag antibodies that have been tested in Simple Western: NBP1-71705, NBP1-97410, and MAB8529.

  • Q: Do FLAG Tag antibodies comes in lyophilized form?

    A: The following FLAG Tag antibodies come in lyophilized form: NBP1-97393, NBP1-97401, NBP1-97399, NBP1-97400, and NBP1-97402.

  • Q: Is this antibody suitable for ChIP analysis of an N-terminally tagged protein?

    A: Our DYKDDDDK epitope tag antibody with catalogue number NB600-344 has been validated for Western blotting, immunofluorescence, immunoprecipitation and ELISA but has not yet been tested for ChIP. However, our DYKDDDDK Epitope Tag antibody with catalog number NBP2-59146 is validated for use in ChIP.

  • Q: What is the immunogen sequence of this FLAG Tag antibody? 

    A: This antibody was made to a ECS enterokinase cleavage site (xxxDDDDK) conjugated to KLH.  

  • Q: What the theoretical molecular weight for FLAG Tag antibodies?

    A: The TMW for DYKDDDDK epitope FLAG Tag is approximately 1.01 kDa.

  • Q: Will FLAG Tag antibodies detect internal FLAG tags? My FLAG sequence is sandwiched between two other protein sequences.

    A: If you are applying your sample(s) to SDS-PAGE, under reducing condition and follow by Western Blot, DYKDDDDK epitope tag antibodies should be able to detect the tag region located internally. However, it depends on the property of your chimeric protein and if your tag is in the hydrophobic or hydrophilic region. Although DYKDDDDK is very hydrophilic, it may not be exposed to the surface if embedded deeply inside the protein with a high content of hydrophobic amino acids with or without covalently-linked modifications such as lipid moieties.

  • Q: Will FLAG Tag antibodies recognize FLAG at the C-terminal end?

    A: Yes, FLAG Tag antibodies can recognize FLAG-tag at both the N- or C-terminal.

  • Q: Are the FLAG Tag antibodies validated in Simple Western?

    A: Yes, we offer 3 FLAG Tag antibodies that have been tested in Simple Western: NBP1-71705, NBP1-97410, and MAB8529.

  • Q: Do FLAG Tag antibodies comes in lyophilized form?

    A: The following FLAG Tag antibodies come in lyophilized form: NBP1-97393, NBP1-97401, NBP1-97399, NBP1-97400, and NBP1-97402.

  • Q: Is this antibody suitable for ChIP analysis of an N-terminally tagged protein?

    A: Our DYKDDDDK epitope tag antibody with catalogue number NB600-344 has been validated for Western blotting, immunofluorescence, immunoprecipitation and ELISA but has not yet been tested for ChIP. However, our DYKDDDDK Epitope Tag antibody with catalog number NBP2-59146 is validated for use in ChIP.

  • Q: What is the immunogen sequence of this FLAG Tag antibody? 

    A: This antibody was made to a ECS enterokinase cleavage site (xxxDDDDK) conjugated to KLH.  

  • Q: What the theoretical molecular weight for FLAG Tag antibodies?

    A: The TMW for DYKDDDDK epitope FLAG Tag is approximately 1.01 kDa.

  • Q: Will FLAG Tag antibodies detect internal FLAG tags? My FLAG sequence is sandwiched between two other protein sequences.

    A: If you are applying your sample(s) to SDS-PAGE, under reducing condition and follow by Western Blot, DYKDDDDK epitope tag antibodies should be able to detect the tag region located internally. However, it depends on the property of your chimeric protein and if your tag is in the hydrophobic or hydrophilic region. Although DYKDDDDK is very hydrophilic, it may not be exposed to the surface if embedded deeply inside the protein with a high content of hydrophobic amino acids with or without covalently-linked modifications such as lipid moieties.

  • Q: Will FLAG Tag antibodies recognize FLAG at the C-terminal end?

    A: Yes, FLAG Tag antibodies can recognize FLAG-tag at both the N- or C-terminal.

  • Q: Are the FLAG Tag antibodies validated in Simple Western?

    A: Yes, we offer 3 FLAG Tag antibodies that have been tested in Simple Western: NBP1-71705, NBP1-97410, and MAB8529.

  • Q: Do FLAG Tag antibodies comes in lyophilized form?

    A: The following FLAG Tag antibodies come in lyophilized form: NBP1-97393, NBP1-97401, NBP1-97399, NBP1-97400, and NBP1-97402.

  • Q: Is this antibody suitable for ChIP analysis of an N-terminally tagged protein?

    A: Our DYKDDDDK epitope tag antibody with catalogue number NB600-344 has been validated for Western blotting, immunofluorescence, immunoprecipitation and ELISA but has not yet been tested for ChIP. However, our DYKDDDDK Epitope Tag antibody with catalog number NBP2-59146 is validated for use in ChIP.

  • Q: What is the immunogen sequence of this FLAG Tag antibody? 

    A: This antibody was made to a ECS enterokinase cleavage site (xxxDDDDK) conjugated to KLH.  

  • Q: What the theoretical molecular weight for FLAG Tag antibodies?

    A: The TMW for DYKDDDDK epitope FLAG Tag is approximately 1.01 kDa.

  • Q: Will FLAG Tag antibodies detect internal FLAG tags? My FLAG sequence is sandwiched between two other protein sequences.

    A: If you are applying your sample(s) to SDS-PAGE, under reducing condition and follow by Western Blot, DYKDDDDK epitope tag antibodies should be able to detect the tag region located internally. However, it depends on the property of your chimeric protein and if your tag is in the hydrophobic or hydrophilic region. Although DYKDDDDK is very hydrophilic, it may not be exposed to the surface if embedded deeply inside the protein with a high content of hydrophobic amino acids with or without covalently-linked modifications such as lipid moieties.

  • Q: Will FLAG Tag antibodies recognize FLAG at the C-terminal end?

    A: Yes, FLAG Tag antibodies can recognize FLAG-tag at both the N- or C-terminal.

Showing  1 - 5 of 7 FAQs Showing All
View all FAQs for Antibodies