Dynactin Subunit 2/DCTN2/DCTN-50 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-30501

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Dynactin Subunit 2/DCTN2 Primary Antibodies »

Product Specifications

Immunogen

between 1 and 50

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Dynactin Subunit 2/DCTN2/DCTN-50 Antibody - BSA Free (NBP3-30501) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Dynactin Subunit 2/DCTN2/DCTN-50 Antibody - BSA Free

Dynactin Subunit 2/DCTN2/DCTN-50 Antibody

Western Blot:[NBP3-30501]-

Western Blot:[NBP3-30501]-Detection of human and mouse DCTN2 by western blot and immunoprecipitation. Samples: Whole cell lysate from Jurkat, HEK293T, HeLa and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-DCTN2 antibody used for WB at 0. 1 µg/ml and 1 µg/ml and used for IP at 6 µg/mg lysate. DCTN2 was also immunoprecipitated by rabbit anti-DCTN2 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds and 3 seconds.

Applications for Dynactin Subunit 2/DCTN2/DCTN-50 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate buffer, pH 7 to 8

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Dynactin Subunit 2/DCTN2

Dynactin subunit 2 encodes a 50-kD subunit of dynactin, a macromolecular complex consisting of 10-11 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit is present in 4-5 copies per dynactin molecule. It contains three short alpha-helical coiled-coil domains that may mediate association with self or other dynactin subunits. It may interact directly with the largest subunit (p150) of dynactin and may affix p150 in place.

Alternate Names

DCTN2, Dynamitin, RBP50

Gene Symbol

DCTN2

Additional Dynactin Subunit 2/DCTN2 Products

Product Documents for Dynactin Subunit 2/DCTN2/DCTN-50 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Dynactin Subunit 2/DCTN2/DCTN-50 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Microtubules

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