E-Tag Epitope Tag Antibody - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-43762
Key Product Details
Species Reactivity
Epitope Tag
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
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Product Specifications
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the E-Tag Epitope Tag sequence (GAPVPYPDPLEPR)
Specificity
Recognizes N-terminal and C-terminal E-Tag Epitope Tag in fusion proteins
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for E-Tag Epitope Tag Antibody - Azide and BSA Free
Western Blot: E-Tag Epitope Tag Antibody [NBP3-43762] -
Western Blot: E-Tag Epitope Tag Antibody [NBP3-43762] - NBP3-43762 at 1:1000 dilution. Lane A: Etag-GFERh(C) transfected 293 cell lysate at 2ug Lane B: Nagetive control at 10ug Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions.
Immunoprecipitation: E-Tag Epitope Tag Antibody [NBP3-43762] -
Immunoprecipitation: E-Tag Epitope Tag Antibody [NBP3-43762] - E-tag was immunoprecipitated using: Lane A:0.5 mg E-tag Whole Cell Lysate 4 uL NBP3-43762 and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: NBP3-43762, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 17 kDa Observed band size :17 kDaApplications for E-Tag Epitope Tag Antibody - Azide and BSA Free
Application
Recommended Usage
Immunoprecipitation
1-5 uL/mg lysate
Western Blot
1:1000-1:5000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
Background: E-Tag Epitope Tag
Alternate Names
E epitope tag, GAPVPYPDPLEPR epitope tag, GAPVPYPDPLEPR tag
Additional E-Tag Epitope Tag Products
Product Documents for E-Tag Epitope Tag Antibody - Azide and BSA Free
Certificate of Analysis
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Product Specific Notices for E-Tag Epitope Tag Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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