E2F-4 Antibody (3O6Y9)

Novus Biologicals | Catalog # NBP3-15850

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3O6Y9 expressed in HEK293
View all E2F-4 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 250-350 of human E2F-4 (Q16254). TAVPGSAEVQGMAGPAAEITVSGGPGTDSKDSGELSSLPLGPTTLDTRPLQSSALLDSSSSSSSSSSSSSNSNSSSSSGPNPSTSFEPIKADPTGVLELPK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for E2F-4 Antibody (3O6Y9)

Western Blot: E2F-4 Antibody (3O6Y9) [NBP3-15850]

Western Blot: E2F-4 Antibody (3O6Y9) [NBP3-15850]

Western Blot: E2F-4 Antibody (3O6Y9) [NBP3-15850] - Western blot analysis of extracts of various cell lines, using E2F-4 antibody (NBP3-15850) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Applications for E2F-4 Antibody (3O6Y9)

Application
Recommended Usage

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: E2F-4

E2F4 is a member of the E2F family of transcription factors. It binds DNA at the E2 recognition site found in the promoter region of a number of genes involved in cell cycle regulation and DNA replication. E2F4 binds to pRb, p107, and p130. Via its interaction with p130, E2F4 plays an important role in maintaining G2 arrest during G2 phase checkpoint activation.

Long Name

E2F Transcription Factor 4

Alternate Names

E2F4

Gene Symbol

E2F4

Additional E2F-4 Products

Product Documents for E2F-4 Antibody (3O6Y9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for E2F-4 Antibody (3O6Y9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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