E2F-4 Antibody (PSH0-45)

Novus Biologicals | Catalog # NBP3-32284

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PSH0-45 expressed in HEK293
View all E2F-4 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within human E2F-4 aa 21-80 / 413. (Uniprot: Q16254)

Localization

Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for E2F-4 Antibody (PSH0-45)

E2F-4 Antibody (PSH0-45)

Western Blot: E2F-4 Antibody (PSH0-45) [NBP3-32284]

Western Blot: E2F-4 Antibody (PSH0-45) [NBP3-32284] - Western blot analysis of E2F-4 on different lysates with Rabbit anti-E2F-4 antibody (NBP3-32284) at 1/1,000 dilution.

Lane 1: 293T cell lysate
Lane 2: Raji cell lysate
Lane 3: HeLa cell lysate
Lane 4: Jurkat cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 44 kDa
Observed band size: 44 kDa

Exposure time: 10 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32284) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Applications for E2F-4 Antibody (PSH0-45)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: E2F-4

E2F4 is a member of the E2F family of transcription factors. It binds DNA at the E2 recognition site found in the promoter region of a number of genes involved in cell cycle regulation and DNA replication. E2F4 binds to pRb, p107, and p130. Via its interaction with p130, E2F4 plays an important role in maintaining G2 arrest during G2 phase checkpoint activation.

Long Name

E2F Transcription Factor 4

Alternate Names

E2F4

Gene Symbol

E2F4

Additional E2F-4 Products

Product Documents for E2F-4 Antibody (PSH0-45)

Certificate of Analysis

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Product Specific Notices for E2F-4 Antibody (PSH0-45)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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