EBI3 Antibody (42N1G4)

Novus Biologicals | Catalog # NBP2-27356

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 42N1G4
View all EBI3 Primary Antibodies »

Product Specifications

Immunogen

Full-length recombinant human EBI3 protein full length recombinant human EBI3 (cat # 100-162)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for EBI3 Antibody (42N1G4)

Western Blot: EBI3 Antibody (42N1G4) [NBP2-27356]

Western Blot: EBI3 Antibody (42N1G4) [NBP2-27356]

Western Blot: IL-27/IL-35 EBI3 Subunit Antibody (42N1G4) [NBP2-27356] - Analysis using EBI3 antibody. Human EBI3 recombinant protein (A) and human IL-35 recombinant protein (B) probed with EBI3 antibody at 5 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Flow Cytometry: EBI3 Antibody (42N1G4) [NBP2-27356]

Flow Cytometry: EBI3 Antibody (42N1G4) [NBP2-27356]

Flow Cytometry: IL-27/IL-35 EBI3 Subunit Antibody (42N1G4) [NBP2-27356] - Intracellular analysis of EBI3 in transfected cells using EBI3 antibody at 0.2 ug/10^6 cells.

Applications for EBI3 Antibody (42N1G4)

Application
Recommended Usage

Flow Cytometry

0.1-1ug/10^6cells

Western Blot

2-5ug/ml~

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EBI3

EBI3 (Epstein-Barr virus Induced-3) is a secreted glycoprotein of the hematopoietin receptor family. It plays a critical regulatory role in the induction of Th2-type immune responses and the development of Th2-mediated tissue inflammation in vivo, which may be mediated through the control of iNkT cell function. EBI3 dimerizes with p28 and p35 subunits of IL-12 to form new proteins IL-27 and IL-35, respectively. IL-27 is an early product of activated antigen presenting cell that is produced upon TLR ligation. It negatively regulates Th17 cell differentiation. EBI3 is widely expressed and its expression in dendritic cell is transcriptionally regulated by TLR signaling via MyD88 and NF-kappaB during innate immune responses preceding cytokine driven Th cell development. EBI3 signal inhibits delayed-type hypersensitivity responses by suppressing IL-17 production and inducing IL-10 hyperproduction. EBI3 may play a novel role in controlling tumor metastasis via lung CD8+ T cells, and its deficiency is associated with a diminished production of Th2 cytokines which are known to regulate allergic airway inflammation in asthma. Targeted deletion of EBI3 protects mice from lung metastasis

Long Name

Epstein-Barr virus induced gene 3

Alternate Names

EBI-3, IL-27 beta, IL-27B, IL-35 EBI3, IL35 EBI3

Gene Symbol

EBI3

UniProt

Q14213 (Human)

Additional EBI3 Products

Product Documents for EBI3 Antibody (42N1G4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for EBI3 Antibody (42N1G4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Regulatory T Cells (Tregs)

Citations for EBI3 Antibody (42N1G4)

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