Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all ECH1 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human ECH1 (Uniprot#: Q13011; Thr34-Leu328)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ECH1 Antibody - Azide and BSA Free

ECH1 Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: ECH1 Antibody [NBP3-00324] -

Immunohistochemistry-Paraffin: ECH1 Antibody [NBP3-00324] - Immunochemical staining of human ECH1 in human prostate with NBP3-00324 (1:10000, formalin-fixed paraffin embedded sections).
ECH1 Antibody - Azide and BSA Free

Western Blot: ECH1 Antibody [NBP3-00324] -

Western Blot: ECH1 Antibody [NBP3-00324] - NBP3-00324 at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:36 kDa Observed band size:34 kDa
ECH1 Antibody - Azide and BSA Free

Immunoprecipitation: ECH1 Antibody [NBP3-00324] -

Immunoprecipitation: ECH1 Antibody [NBP3-00324] - ECH1 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL NBP3-00324 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00324, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 34 kDa Observed band size: 34 kDa
ECH1 Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: ECH1 Antibody [NBP3-00324] -

Immunohistochemistry-Paraffin: ECH1 Antibody [NBP3-00324] - Immunochemical staining of human ECH1 in human liver(cirrhosis) with NBP3-00324 (1:10000, formalin-fixed paraffin embedded sections).Positive staining was localized to cytoplasm of hepatocyte

Applications for ECH1 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunohistochemistry-Paraffin

1:2500-1:10000

Immunoprecipitation

0.5-2 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ECH1

ECH1 encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.

Alternate Names

delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial, delta3,5-delta2,4-dienoyl-CoA isomerase, dienoyl-CoA isomerase, EC 5.3.3.-, enoyl CoA hydratase 1, peroxisomal, enoyl Coenzyme A hydratase 1, peroxisomal, HPXEL, peroxisomal enoyl-CoA hydratase 1

Gene Symbol

ECH1

Additional ECH1 Products

Product Documents for ECH1 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for ECH1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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