EEF2K Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-12694

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EEF2K Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human EEF2K fragment

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Protein A & Antigen Affinity purified

Scientific Data Images for EEF2K Antibody - BSA Free

Western Blot: EEF2K Antibody [NBP3-12694]

Western Blot: EEF2K Antibody [NBP3-12694]

Western Blot: EEF2K Antibody [NBP3-12694] - Lane A: A431 Whole Cell LysateLane B: H1299 Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:82 kDa
Immunoprecipitation: EEF2K Antibody [NBP3-12694]

Immunoprecipitation: EEF2K Antibody [NBP3-12694]

Immunoprecipitation: EEF2K Antibody [NBP3-12694] - Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-EEF2K rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-EEF2K rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 82 kDaObserved band size :100 kDa

Applications for EEF2K Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1-5ul/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EEF2K

EEF2K encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq]

Alternate Names

Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase, EC 2.7.11, EC 2.7.11.20, eEF-2 kinase, eEF-2Kcalmodulin-dependent protein kinase III, elongation factor-2 kinase, eukaroytic elongation factor 2 kinase, eukaryotic elongation factor 2 kinase, eukaryotic elongation factor-2 kinase, HSU93850, MGC45041

Gene Symbol

EEF2K

Additional EEF2K Products

Product Documents for EEF2K Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for EEF2K Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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