Eg5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84887

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Eg5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ALIKEYTEEIERLKRDLAAAREKNGVYISEENFRVMSGKLTVQEEQIVELIEKIGAVEEELNRVTELFMDNKNELDQCKSDLQNKTQELETTQKHLQETKLQLVK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Eg5 Antibody - BSA Free

Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84887]

Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84887]

Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84887] - Analysis in human tonsil and pancreas tissues. Corresponding KIF11 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84887]

Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84887]

Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84887] - Staining of human tonsil shows moderate to strong cytoplasmic positivity, mainly in germinal center cells.

Western Blot: Eg5 Antibody [NBP1-84887] -

Western Blot: Eg5 Antibody [NBP1-84887] - Analysis in human cell line A-431.
Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84887]

Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84887]

Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84887] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84887]

Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84887]

Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84887] - Staining of human duodenum shows moderate to strong cytoplasmic positivity in a subset of glandular cells.
Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84887]

Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84887]

Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84887] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Applications for Eg5 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Eg5

Eg5 is a mitotic motor protein required for chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. Eg-5 is therefore crucial for proper chromosome segregation during cell division. Eg 5 antibodies are useful tools for cell cycle studies and research on mitotic spindles.

Long Name

Kinesin-like protein KIF11

Alternate Names

KIF11, KNSL1, TRIP-5, TRIP5

Gene Symbol

KIF11

Additional Eg5 Products

Product Documents for Eg5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Eg5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Eg5 Antibody - BSA Free

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Protocols

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FAQs

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