Recombinant Human EGF Protein

Name: Recombinant Human EGF Protein
Brand: Novus Biologicals
SKU: NBP2-61377
Price: 332 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-61377

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Source

Yeast

Applications

Western Blot, Bioactivity

View all EGF Proteins and Enzymes »

Product Specifications

Description

A single glycosylated polypeptide chain containing 51 amino acids corresponding to EGF Source: Yeast

Uniprot ID: P01133

Amino Acid Sequence: NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW E

Purity

>98%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of EGF as determined by LAL method.

Predicted Molecular Mass

6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 x 10^7 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human EGF Protein

SDS-PAGE: Recombinant Human EGF Protein [NBP2-61377]

SDS-Page: Recombinant Human EGF Protein [NBP2-61377]

SDS-PAGE: Recombinant Human EGF Protein [NBP3-18003] -

SDS-Page of Recombinant Human EGF Protein

Formulation, Preparation, and Storage

NBP2-61377

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: EGF

Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.

Long Name

Epidermal Growth Factor

Alternate Names

HOMG4, URG, Urogastrone

Gene Symbol

EGF

Additional EGF Products

Product Documents for Recombinant Human EGF Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Human EGF Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.