ELF3/ESE-1 Antibody (JE33-30)

Western Blot: ELF3/ESE-1 Antibody (JE33-30) [NBP3-32320]

Western Blot: ELF3/ESE-1 Antibody (JE33-30) [NBP3-32320] - Western blot analysis of ELF3/ESE-1 on different lysates with Rabbit anti-ELF3/ESE-1 antibody (NBP3-32320) at 1/1,000 dilution.

Lane 1: A549 cell lysate (10 ug/Lane)
Lane 2: SK-Br-3 cell lysate (10 ug/Lane)
Lane 3: A431 cell lysate (10 ug/Lane)
Lane 4: Mouse lung tissue lysate (20 ug/Lane)
Lane 5: Rat kidney tissue lysate (20 ug/Lane)

Predicted band size: 41.5 kDa
Observed band size: 41.5 kDa

Exposure time: 40 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32320) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Flow Cytometry: ELF3/ESE-1 Antibody (JE33-30) [NBP3-32320]

Flow Cytometry: ELF3/ESE-1 Antibody (JE33-30) [NBP3-32320] - Flow cytometric analysis of A431 cells labeling ELF3/ESE-1.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32320, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).