Elk-1 Antibody (2G6) - Azide and BSA Free

Novus Biologicals | Catalog # H00002002-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2G6

Format

Azide and BSA Free
View all Elk-1 Primary Antibodies »

Product Specifications

Immunogen

ELK1 (NP_005220, 67 a.a. ~ 166 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. YYDKNIIRKVSGQKFVYKFVSYPEVAGCSTEDCPPQPEVSVTSTMPNVAPAAIHAAPGDTVSGKPGTPKGAGMAGPGGLARSSRNEYMRSGLYSTFTIQS

Specificity

ELK1 - ELK1, member of ETS oncogene family

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Elk-1 Antibody (2G6) - Azide and BSA Free

Proximity Ligation Assay: Elk-1 Antibody (2G6) [H00002002-M01]

Proximity Ligation Assay: Elk-1 Antibody (2G6) [H00002002-M01]

Proximity Ligation Assay: Elk-1 Antibody (2G6) [H00002002-M01] - Analysis of protein-protein interactions between MAPK3 and ELK1. HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-ELK1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Sandwich ELISA: Elk-1 Antibody (2G6) [H00002002-M01] - Detection limit for recombinant GST tagged ELK1 is approximately 1ng/ml as a capture antibody.

Applications for Elk-1 Antibody (2G6) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Proximity Ligation Assay

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against recombinant protein on ELISA. GST alone used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Elk-1

This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Alternate Names

Elk1

Entrez Gene IDs

2002 (Human)

Gene Symbol

ELK1

OMIM

311040 (Human)

UniProt

NP_005220 (Human)

Additional Elk-1 Products

Product Documents for Elk-1 Antibody (2G6) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Elk-1 Antibody (2G6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Elk-1 Antibody (2G6) - Azide and BSA Free

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FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Adipokines & Insulin Signaling Pathways
Adipokines & Insulin Signaling Pathway Thumbnail