ELOVL5 Antibody (JE65-70)

Novus Biologicals | Catalog # NBP3-32305

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE65-70 expressed in HEK293
View all ELOVL5 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within human ELOVL5 aa 250-299/299. (Uniprot: Q9NYP7)

Localization

Endoplasmic reticulum membrane, dendrite.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ELOVL5 Antibody (JE65-70)

ELOVL5 Antibody (JE65-70)

Western Blot: ELOVL5 Antibody (JE65-70) [NBP3-32305]

Western Blot: ELOVL5 Antibody (JE65-70) [NBP3-32305] - Western blot analysis of ELOVL5 on different lysates with Rabbit anti-ELOVL5 antibody (NBP3-32305) at 1/500 dilution.

Lane 1: Rat kidney tissue lysate
Lane 2: Rat brain tissue lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 35 kDa
Observed band size: 38 kDa

Exposure time: 2 minutes;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32305) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Applications for ELOVL5 Antibody (JE65-70)

Application
Recommended Usage

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ELOVL5

ELOVL5 plays a role in elongation of long-chain polyunsaturated fatty acids (Leonard et al., 2000)

Alternate Names

dJ483K16.1, EC 2.3.1.n8, elongation of very long chain fatty acids protein 5, ELOVL family member 5, elongation of long chain fatty acids (FEN1/Elo2, ELOVL2,3-keto acyl-CoA synthase ELOVL5, Fatty acid elongase 1, hELO1, HELO1homolog of yeast long chain polyunsaturated fatty acid elongation enzyme 2, homolog of yeast long chain polyunsaturated fatty acid elongatio, SUR4/Elo3-like, yeast)

Gene Symbol

ELOVL5

Additional ELOVL5 Products

Product Documents for ELOVL5 Antibody (JE65-70)

Certificate of Analysis

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Product Specific Notices for ELOVL5 Antibody (JE65-70)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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