EMAP-II/AIMP1 Antibody

Novus Biologicals | Catalog # NBP2-27080

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Chinese Hamster, Equine, Guinea Pig, Primate

Predicted:

Chicken (94%), Chimpanzee (100%), Orangutan (100%), Rabbit (94%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all EMAP-II Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 260-310 from human AIMP1 was used as the immunogen for this antibody.

Reactivity Notes

The amino acid sequence used as immunogen for this antibody is 100% homologous in Rhesus Macaque, White-cheeked Gibbon, 89% in New World Monkey, Canine, Opossum, Feline, Frog, Zebrafish.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit EMAP-II/AIMP1 Antibody (NBP2-27080) is a polyclonal antibody validated for use in WB and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for EMAP-II/AIMP1 Antibody

Western Blot: EMAP-II/AIMP1 Antibody [NBP2-27080]

Western Blot: EMAP-II/AIMP1 Antibody [NBP2-27080]

Western Blot: EMAP-II/AIMP1 Antibody [NBP2-27080] - Analysis of AIMP1 using AIMP1 antibody. Human HCT116 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) mouse Raw 4) 3T3 cell lysate probed with 2 ug/ml of AIMP1 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Simple Western: EMAP-II/AIMP1 Antibody [NBP2-27080]

Simple Western: EMAP-II/AIMP1 Antibody [NBP2-27080]

Simple Western: EMAP-II/AIMP1 Antibody [NBP2-27080] - Simple Western lane view shows a specific band for EMAP-II/AIMP1 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for EMAP-II/AIMP1 Antibody

Application
Recommended Usage

Simple Western

1:50

Western Blot

2-4ug/ml
Application Notes

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in NIH-3T3 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 48 kDa.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EMAP-II

AIMP1, short for Aminoacyl tRNAse synthetase complex iVinteracting multifunctional protein 1, is also called p43 (~43 kDa molecular weight). Along with AIMP2 and AIMP3, AIMP1 is one of the three auxiliary proteins which bind to the aminoacyl tRNase synthetase complex. The AIMPs together with the Aminoacyl tRNAse synthetase, ARS, complex perform a critical role in protein synthesis but also in biological processes such as apoptosis, angiogenesis and inflammation (1, 2). AIMP1 is characterized as a pleiotropic cytokine that enhances TLR1, TLR2, TLR3 and TLR7 expression in BM-DCs. AIMP1 also enhances DC production of IL-6 and IL-12 and increases NF-fUB activity (3, 4). AIMP1 has demonstrated to immune-stimulating activities as well as anti-tumor activities in mouse xenograft models (5). Expression AIMP1 and AIMP2 and 3 proteins are diminished in certain cancers (6). Overall, AIMPs may act as tumor suppressors and perhaps may be considered as a focused target for cancer treatment candidates.

Long Name

Endothelial-Monocyte Activating Polypeptide II

Alternate Names

AIMP1, EMAPII, SCYE1

Gene Symbol

AIMP1

Additional EMAP-II Products

Product Documents for EMAP-II/AIMP1 Antibody

Certificate of Analysis

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Download SDS

Product Specific Notices for EMAP-II/AIMP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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