endo-IWR 1

Key Product Details

Product Description

endo

-IWR 1 is an inhibitor of Wnt signaling. Induces an increase in axin2 protein levels; promotes β-catenin phosphorylation by stabilizing Axin-scaffolded destruction complexes. Promotes endothelial cell specification of cardiac progenitor cells and may be used in protocols for the generation of retinal pigment epithelial cells from hPSCs, and for the generation and propagation of EPS cells (see our protocol below).

Negative Control also available.

endo-IWR 1 synthesized to Ancillary Material Grade also available.

For more information about how endo-IWR 1 may be used, see our protocol: Generation and Propagation of EPS cells (LCDM Cocktail).

Product Specifications for endo-IWR 1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for endo-IWR 1

The following data is based on the product molecular weight 409.44.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

2 1 Recalculate

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.44 mL	12.21 mL	24.42 mL
5 mM	0.49 mL	2.44 mL	4.88 mL
10 mM	0.24 mL	1.22 mL	2.44 mL
50 mM	0.05 mL	0.24 mL	0.49 mL

Calculators

Background References

References are publications that support the biological activity of the product. See our Citations tab to view 61 publications citing the usage of this product.

• Surendran An improved protocol for generation and characterization of human-induced pluripotent stem cell-derived retinal pigment epithelium cells. STAR Protoc. 2022 PMID: 36386870
• Reichman Wnt inhibition promotes vascular specification of embryonic cardiac progenitors. Development 2018 PMID: 29217753
• Lancaster Generation of cerebral organoids from human pluripotent stem cells. Nat.Protoc. 2015 PMID: 25188634
• Kadoshima Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell-derived neocortex. Proc.Natl.Acad.Sci.USA 2013 PMID: 24277810
• Chen Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nature Chem.Biol. 2008 PMID: 19125156
• Lu Structure-activity relationship studies of small-molecule inhibitors of Wnt response. Bioorg.Med.Chem.Lett. 2009 PMID: 19410457

Product Documents for endo-IWR 1