Recombinant Human ENO3 His Protein

Novus Biologicals | Catalog # NBP1-72501

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

Enzyme Activity, SDS-PAGE
View all ENO3 Proteins and Enzymes »

Product Specifications

Description

A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-434 of Human ENO3

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAMQKIFARE ILDSRGNPTV EVDLHTAKGR FRAAVPSGAS TGIYEALELR DGDKGRYLGK GVLKAVENIN STLGPALLQK KLSVADQEKV DKFMIELDGT ENKSKFGANA ILGVSLAVCK AGAAEKGVPL YRHIADLAGN PDLILPVPAF NVINGGSHAG NKLAMQEFMI LPVGASSFKE AMRIGAEVYH HLKGVIKAKY GKDATNVGDE GGFAPNILEN NEALELLKTA IQAAGYPDKV VIGMDVAASE FYRNGKYDLD FKSPDDPARH ITGEKLGELY KSFIKNYPVV SIEDPFDQDD WATWTSFLSG VNIQIVGDDL TVTNPKRIAQ AVEKKACNCL LLKVNQIGSV TESIQACKLA QSNGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEALGDK AIFAGRKFRN PKAK

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Specific activity is > 5,000pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37C.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human ENO3 His Protein

Recombinant Human ENO3 Protein [NBP1-72501]

Formulation, Preparation, and Storage

NBP1-72501
Formulation 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 20% glycerol, 0.1 M NaCl
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ENO3

Beta-enolase, also known as ENO3, is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. ENO3 play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. Mutations in its gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Recombinant human ENO3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

2-phospho-D-glycerate hydrolyase, 2-phospho-D-glycerate hydro-lyase, beta-enolase, EC 4.2.1, EC 4.2.1.11, Enolase 3, enolase 3 (beta, muscle), enolase 3, (beta, muscle), GSD13, MSE, Muscle-specific enolase, Skeletal muscle enolase

Gene Symbol

ENO3

Additional ENO3 Products

Product Documents for Recombinant Human ENO3 His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human ENO3 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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Protocols

View specific protocols for Recombinant Human ENO3 His Protein (NBP1-72501):

SDS-PAGE (NBP1-72501):
1. Prepare 1,450ul assay buffer. The final concentrations are 81 mM Triethanolamine, 1.9 mM 2-phosphorglycerate, 0.12 mM beta- NADPH, 25 mM magnesium sulfate, 100 mM potassium chloride, 1.3 mM ADP, 4 unit pyruvate kinase, 6 unit L-lactic dehydrogenas.


2. Add 50ul of recombinant beta-enolase protein in various concentrations (0.25 ug, 0.5 ug, 0.1 ug) in assay buffer.


3. Mix by inversion and load 200 ul of reaction mix in to a plate well.


4. Record the decrease in A340 nm for 5 minutes at 25C.

FAQs

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