Enolase 1 Antibody (A6)

Novus Biologicals | Catalog # NBP3-27947

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # A6 expressed in HEK293
View all Enolase 1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptides derived from human Enolase 1

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for Enolase 1 Antibody (A6)

Enolase 1 Antibody (A6)

Western Blot: Enolase 1 Antibody (A6) [NBP3-27947] -

Western Blot: Enolase 1 Antibody (A6) [NBP3-27947] - Antibody: Enolase 1 at 1/1000 dilution
Sample: NIH-3T3 at 30ug
Secondary: HRP conjugated anti-Rabbit Fc at 1/50000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Gel concentration: 4-20%

Applications for Enolase 1 Antibody (A6)

Application
Recommended Usage

Western Blot

1:1000 – 1:5000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2), 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Enolase 1

Enolase 1 encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.

Alternate Names

2-phospho-D-glycerate hydro-lyase, Alpha enolase, C-myc promoter-binding protein, c-myc promoter-binding protein-1, EC 4.2.1, EC 4.2.1.11, ENO1L1, Enolase 1, enolase 1, (alpha), MBP-1, MBPB1, MPB-1, MPB1c-myc promoter-binding protein 1, MYC promoter-binding protein 1, NNE, Non-neural enolase, Phosphopyruvate hydratase, Plasminogen-binding protein, PPHalpha enolase like 1, tau-crystallin

Gene Symbol

ENO1

UniProt

P06733 (Human)

Additional Enolase 1 Products

Product Documents for Enolase 1 Antibody (A6)

Certificate of Analysis

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Product Specific Notices for Enolase 1 Antibody (A6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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