Enolase 2/Neuron-specific Enolase Antibody (5D3) - BSA Free

Novus Biologicals | Catalog # NBP2-37465

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5D3

Format

BSA Free
View all Enolase 2/Neuron-specific Enolase Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Enolase 2/Neuron-specific Enolase (AA: 251-433) expressed in E. Coli.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

47.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Enolase 2/Neuron-specific Enolase Antibody (5D3) - BSA Free

Western Blot: Enolase 2/Neuron-specific Enolase Antibody (5D3)BSA Free [NBP2-37465]

Western Blot: Enolase 2/Neuron-specific Enolase Antibody (5D3)BSA Free [NBP2-37465]

Western Blot: Enolase 2/Neuron-specific Enolase Antibody (5D3) [NBP2-37465] - Analysis using ENO2 mouse mAb against Mouse brain (1), NIH3T3 (2), and C6 (3) cell lysate.
Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (5D3) - BSA Free [NBP2-37465]

Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (5D3) - BSA Free [NBP2-37465]

Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (5D3) [NBP2-37465] - Analysis of liver cancer tissues using ENO2 mouse mAb with DAB staining.
Flow Cytometry: Enolase 2/Neuron-specific Enolase Antibody (5D3) - BSA Free [NBP2-37465]

Flow Cytometry: Enolase 2/Neuron-specific Enolase Antibody (5D3) - BSA Free [NBP2-37465]

Flow Cytometry: Enolase 2/Neuron-specific Enolase Antibody (5D3) [NBP2-37465] - Analysis of HeLa cells using ENO2 mouse mAb (green) and negative control (red).
Western Blot: Enolase 2/Neuron-specific Enolase Antibody (5D3)BSA Free [NBP2-37465]

Western Blot: Enolase 2/Neuron-specific Enolase Antibody (5D3)BSA Free [NBP2-37465]

Western Blot: Enolase 2/Neuron-specific Enolase Antibody (5D3) [NBP2-37465] - Analysis using ENO2 mAb against human ENO2 recombinant protein. (Expected MW is 46 kDa)
Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (5D3) - BSA Free [NBP2-37465]

Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (5D3) - BSA Free [NBP2-37465]

Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (5D3) [NBP2-37465] - Analysis of lung cancer tissues using ENO2 mouse mAb with DAB staining.
ELISA: Enolase 2/Neuron-specific Enolase Antibody (5D3) - BSA Free [NBP2-37465]

ELISA: Enolase 2/Neuron-specific Enolase Antibody (5D3) - BSA Free [NBP2-37465]

ELISA: Enolase 2/Neuron-specific Enolase Antibody (5D3) [NBP2-37465] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for Enolase 2/Neuron-specific Enolase Antibody (5D3) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Enolase 2/Neuron-specific Enolase

Neuron-specific enolase (NSE) is a glycolytic isoenzyme which is located in central and peripheral neurons and neuroendocrine cells. This enzyme is released into the CSF when neural tissue is injured. Neoplasms derived from neural or neuroendocrine tissue may release NSE into the blood. NSE is a useful substance that has been detected in patients with certain tumors, namely: neuroblastoma, small cell lung cancer, medullary thyroid cancer, carcinoid tumors, pancreatic endocrine tumors, and melanoma. Recombinant NSE was expressed in E.coli and purified by conventional chromatography techniques.

Alternate Names

ENO2, gamma-Enolase, Neuronspecific Enolase, NSE

Gene Symbol

ENO2

Additional Enolase 2/Neuron-specific Enolase Products

Product Documents for Enolase 2/Neuron-specific Enolase Antibody (5D3) - BSA Free

Certificate of Analysis

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Product Specific Notices for Enolase 2/Neuron-specific Enolase Antibody (5D3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Neuronal Markers

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Protocols

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