Enolase 2/Neuron-specific Enolase Antibody

Novus Biologicals | Catalog # NB110-58870

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Knockdown Validated

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Enolase 2/Neuron-specific Enolase Primary Antibodies »

Product Specifications

Immunogen

Recombinant full length human NSE purified from E. coli. [UniProt# P09104]

Marker

Neuronal Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Enolase 2/Neuron-specific Enolase Antibody

Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870]

Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870]

Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Analysis of different tissue and cell lysates using rabbit pAb to neuron specific enolase (NSE), NB110-58870, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] NIH-3T3, [7] HEK293, [8] HeLa, [9] SH-SY5Y, and [10] C6 cells. A single band at about 47kDa corresponds to the NSE protein, seen only in extracts containing neurons ro neuronal lineage cells.
Immunocytochemistry/ Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870]

Immunocytochemistry/ Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870]

Immunocytochemistry/Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Analysis of mixed cortical neuron-glial cell culture from E20 rat stained with rabbit pAb to neuron specific enolase (NSE), NB110-58870, dilution 1:500 in red, and costained with chicken pAb to GFAP, dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. the NSE antibody labels protein expressed in neuronal cells, while the GFAP antibody stains intermediate filaments in astrocytic and certain other glial cells.
Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870]

Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870]

Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Blot of rat spinal cord probed with rabbit antibody to NSE. The antibody stains a single sharp band corresponing to NSE at about 47kDa.
Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870]

Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870]

Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Western blot analysis of extracts from mouse brain using NSE antibody (NB110-58870, 1:100).
Immunocytochemistry/ Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870]

Immunocytochemistry/ Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870]

Immunocytochemistry/Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Shows the human embryonic kidney cells line 293, which express many neuronal proteins (1). The red channel shows staining which recognizes all of these 293 cells. The green channels shows staining for another neuronal marker to UCHL1 using NB110-58869. This neuronal gene is apparently activated in a cell density dependent fashion and at this stage only a few cells express this protein. However all cells that express NSE also express UCHL1.
Immunocytochemistry/ Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870]

Immunocytochemistry/ Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870]

Immunocytochemistry/Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Confocal immunofluorescent analysis of 3T3 cells using NSE antibody (NB110-58870, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).
Simple Western: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870]

Simple Western: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870]

Simple Western: Enolase 2/Neuron-specific Enolase Antibody [NB110-58870] - Simple Western lane view shows a specific band for Enolase 2/ Neuron specific enolase in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for Enolase 2/Neuron-specific Enolase Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Immunohistochemistry

1:500

Simple Western

1:10000

Western Blot

1:2000
Application Notes
This NSE antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Human Brain lysate 0.05 mg/mL, separated by Size, antibody dilution of 1:10,000. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 2 reviews rated 5 using NB110-58870 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Supplied as serum

Preservative

0.035% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Enolase 2/Neuron-specific Enolase

NSE (neuron-specific enolase or enolase 2) is an enzyme which facilitates the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolytic pathway, and also the reverse reaction in gluconeogenic metabolism. It belongs to mammalian enolases ENO1, ENO2, and ENO3 (alternately known as enolase alpha, beta and gamma respectively) and these three enolases have different cell type specific expression patterns. NSE is found in relatively large amounts in neurons, PNS tissues and neuroendocrine cells, and is one of the most commonly employed biochemical markers of nervous tissue damage. Increased NSE levels in serum, CSF, or both have been reported in several neurological pathologies such as status epilepticus, Creutzfeldt-Jakob disease, acute ischemic stroke, head injury and hypoxic brain injury and Tuberculous Meningitis.

Alternate Names

ENO2, gamma-Enolase, Neuronspecific Enolase, NSE

Entrez Gene IDs

2026 (Human); 24334 (Rat)

Gene Symbol

ENO2

UniProt

P09104

Additional Enolase 2/Neuron-specific Enolase Products

Product Documents for Enolase 2/Neuron-specific Enolase Antibody

Certificate of Analysis

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Product Specific Notices for Enolase 2/Neuron-specific Enolase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Neuronal Markers

Citations for Enolase 2/Neuron-specific Enolase Antibody

Customer Reviews for Enolase 2/Neuron-specific Enolase Antibody (2)

5 out of 5
2 Customer Ratings
5 Stars
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1 Stars
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  • Name: Bryan Tinsley
    Application: Immunocytochemistry
    Sample Tested:
    Species: Mouse
    Verified Customer | Posted 06/05/2014
    Confocal immunofluorescent analysis of 3T3 cells using NSE antibody (NB110-58870, 1:5).
    Enolase 2/Neuron-specific Enolase Antibody NB110-58870
  • Name: Bryan Tinsley
    Application: Western Blot
    Sample Tested:
    Species: Mouse
    Verified Customer | Posted 06/05/2014
    Western blot analysis of extracts from mouse brain using NSE antibody (NB110-58870, 1:100).
    Enolase 2/Neuron-specific Enolase Antibody NB110-58870

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