ENPP-2/Autotaxin Antibody

Name: ENPP-2/Autotaxin Antibody
Brand: Novus Biologicals
SKU: NBP2-16339
Price: 489 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-16339

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Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (95%), Chicken (95%), Mouse (95%), Rat (95%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human ENPP-2/Autotaxin. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (87%).

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

99 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ENPP-2/Autotaxin Antibody

Western Blot: ENPP-2/Autotaxin Antibody [NBP2-16339]

Western Blot: ENPP-2/Autotaxin Antibody [NBP2-16339] - Human cerebrospinal fluid (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Autotaxin antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/ Immunofluorescence: ENPP-2/Autotaxin Antibody [NBP2-16339] -

Autotaxin antibody detects Autotaxin protein at cytoplasm by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in ice-cold MeOH for 5 min.
Green: Autotaxin protein stained by Autotaxin antibody (NBP2-16339) diluted at 1:500.
Blue: Hoechst 33342 staining.

Applications for ENPP-2/Autotaxin Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ENPP-2/Autotaxin

The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Long Name

Ectonucleotide Pyrophosphatase/Phosphodiesterase 2

Alternate Names

ATX, Autotaxin, ENPP2, Lysophosphatidic Acid, NPP2, PDNP2

Gene Symbol

ENPP2

UniProt

Q13822

Additional ENPP-2/Autotaxin Products

Product Documents for ENPP-2/Autotaxin Antibody

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Certificate of Analysis

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Product Specific Notices for ENPP-2/Autotaxin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Phosphatases and Regulators

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