ENT1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-32223

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ENT1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 343 to 393 of human Equilibrative NBMPR-Sensitive Nucleoside Transporter using the numbering given in entry NP_001071645.1 (GeneID 2030).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ENT1 Antibody - BSA Free

Immunoprecipitation: ENT1 Antibody [NBP2-32223]

Immunoprecipitation: ENT1 Antibody [NBP2-32223]

Immunoprecipitation: ENT1 Antibody [NBP2-32223] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-ENT1 antibody NBP2-32223 used for IP at 6 ug per reaction. For blotting immunoprecipitated ENT1 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for ENT1 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ENT1

ENT1 is a member of the equilibrative nucleoside transporter family. The gene encodes a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylthioinosine (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq]

Long Name

Equilibrative nucleoside transporter 1

Alternate Names

SLC29A1

Gene Symbol

SLC29A1

UniProt

NP_001071645.1 (Human)

Additional ENT1 Products

Product Documents for ENT1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for ENT1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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