Epac2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-32009

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rat (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Epac2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: VIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDNCHFLRVDKEDFNRILRDVEANTVRLKEHDQDVLVLEKVPAGNRASNQGNSQPQQKYTVMSGTPEKILEHFLETIRLEATLNEATDSVLNDFIMMHCVFMPNTQLC

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Epac2 Antibody - BSA Free

Western Blot: Epac2 Antibody [NBP2-32009]

Western Blot: Epac2 Antibody [NBP2-32009]

Western Blot: Epac2 Antibody [NBP2-32009] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: Epac2 Antibody [NBP2-32009]

Immunocytochemistry/ Immunofluorescence: Epac2 Antibody [NBP2-32009]

Immunocytochemistry/Immunofluorescence: Epac2 Antibody [NBP2-32009] - Staining of human cell line RT4 shows localization to focal adhesion sites. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Epac2 Antibody [NBP2-32009]

Immunohistochemistry-Paraffin: Epac2 Antibody [NBP2-32009]

Immunohistochemistry-Paraffin: Epac2 Antibody [NBP2-32009] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Applications for Epac2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 3 using NBP2-32009 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Epac2

Epac2, also known as Rap Guanine Nucleotide Exchange Factor (GEF) 4, is a guanine nucleotide exchange factor for RAP1A, RAP1B and RAP2A small GTPases. Studies have shown that EPAC2 has a significant role in the development of type 2 diabetes. EPAC2 has also been studied in relation to ataxia, neuronitis, osteoporosis and pancreatitis.

Long Name

Rap guanine nucleotide exchange factor 4

Alternate Names

cAMP-GEFII, CGEF2, EPAC 2, RAPGEF4

Gene Symbol

RAPGEF4

UniProt

Q8WZA2 (Human)

Additional Epac2 Products

Product Documents for Epac2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Epac2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Epac2 Antibody - BSA Free (1)

3 out of 5
1 Customer Rating
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Customer Images

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  • Epac2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Min6 mouse insulinoma whole cell lysate
    Species: Mouse
    Verified Customer | Posted 04/25/2019
    Western Blot: Epac2 Ab [NBP2-32009] - Analysis of MIN6 cell lysates. Lane 1: Molecular Weight Ladder. Lanes 2-5: MIN6 cells (~10 ug). Primary Antibody: Anti-Epac2 Ab at 1:750 overnight at 4C. Secondary Antibody: Goat Anti-rabbit IgG: HRP at 1:3000 for 1hr RT.
    Epac2 Antibody - BSA Free NBP2-32009

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Protocols

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FAQs

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