EphA2 Antibody (M088)

Novus Biologicals | Catalog # NBP3-23321

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # M088
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Product Specifications

Immunogen

Clone (M088) was generated from a recombinant protein that included the extracellular region of human EphA2 protein. [UniProt# P29317]

Specificity

Clone M088 mouse monoclonal antibody detects native human EphA2, as well as aldehyde-fixed EphA2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for EphA2 Antibody (M088)

EphA2 Antibody (M088)

ELISA: EphA2 Antibody (M088) [NBP3-23321] -

ELISA: EphA2 Antibody (M088) [NBP3-23321] - Representative Standard Curve using mouse monoclonal anti-EphA2 (NBP3-23321) for ELISA capture of human recombinant EphA2 extracellular region with a His-tag. Captured protein was detected by suitable anti-His-tag antibody followed by appropriate secondary antibody HRP conjugate.
EphA2 Antibody (M088)

Immunocytochemistry/Immunofluorescence: EphA2 Antibody (M088) [NBP3-23321] -

Immunocytochemistry/Immunofluorescence: EphA2 Antibody (M088) [NBP3-23321] - Immunocytochemical labeling of EphA2 in aldehyde fixed human MDA-MB-231 breast carcinoma cells. The cells were labeled with mouse monoclonal anti-EphA2 (NBP3-23321). The antibody was detected using goat anti-mouse DyLight(R) 594.

Applications for EphA2 Antibody (M088)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200

Immunoprecipitation

1:100

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: EphA2

Eph receptor A2 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands.

Alternate Names

Eck, Myk2, Sek2

Gene Symbol

EPHA2

Additional EphA2 Products

Product Documents for EphA2 Antibody (M088)

Certificate of Analysis

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Product Specific Notices for EphA2 Antibody (M088)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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