Ephrin-B1 [p Tyr331] Antibody - Azide Free

Novus Biologicals | Catalog # NB300-276

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Bovine (100%), Chicken (100%), Human (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all Ephrin-B1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ephrin-B1 conjugated to KLH. Accession # P28693

Modification

p Tyr331

Specificity

Specific for endogenous levels of the ~46 kDa Ephrin-B1 protein phosphorylated at Tyr331. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ephrin-B1 [p Tyr331] Antibody - Azide Free

Western Blot: Ephrin-B1 [p Tyr331] Antibody [NB300-276] - Analysis of EphB1 [p Tyr331] Antibody in rat testes lysate using this antibody at 1:1000.

Applications for Ephrin-B1 [p Tyr331] Antibody - Azide Free

Application
Recommended Usage

Western Blot

1:1000
Application Notes
This antibody has been tested in Western blot on rat lysate, detecting a band ~46 kDa.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ephrin-B1

EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event. Recent work suggests that phosphorylation of a specific EphrinB residue (Tyr298) plays a key role in EphrinB signaling. (3, 5)

Alternate Names

Cek5-L, EFL-3, EFNB1, ELK-L, EphrinB1, LERK-2, STRA-1

Gene Symbol

EFNB1

Additional Ephrin-B1 Products

Product Documents for Ephrin-B1 [p Tyr331] Antibody - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Ephrin-B1 [p Tyr331] Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Ephrin Ligands and Eph Receptors

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