Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunoprecipitation, Western Blot (Negative)

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EPLIN Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 500 and 550 of human epithelial protein lost in neoplasm beta using the numbering given in entry NP_057441.1 (GeneID 51474).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit EPLIN Antibody - BSA Free (NB100-2310) is a polyclonal antibody validated for use in IP. Anti-EPLIN Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for EPLIN Antibody - BSA Free

Immunoprecipitation: EPLIN Antibody [NB100-2310]

Immunoprecipitation: EPLIN Antibody [NB100-2310]

Immunoprecipitation: EPLIN Antibody [NB100-2310] - Detection of human EPLIN by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EPLIN antibody NB100-2310 (lot NB100-2310-2) used for IP at 6 ug per reaction. EPLIN was also immunoprecipitated by a previous lot of this antibody (lot NB100-2310-1) and rabbit anti-EPLIN antibody NB100-2305. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for EPLIN Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate
Application Notes
Not Recommended for Western Blot. Use rabbit anti-EPLIN antibody NB100-2305

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: EPLIN

EPLIN is a cytoskeleton associated protein that inhibits actin filament depolymerization and crosslinks filaments in bundles.

Alternate Names

1110021C24Rik, Epithelial protein lost in neoplasm, EPLINepithelial protein lost in neoplasm beta, FLJ38853, LIM domain and actin binding 1, MGC131726, SREBP3LIM domain and actin-binding protein 1, sterol regulatory element binding protein 3

Entrez Gene IDs

51474 (Human); 65970 (Mouse)

Gene Symbol

LIMA1

UniProt

Q9UHB6 (Human)

Additional EPLIN Products

Product Documents for EPLIN Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for EPLIN Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for EPLIN Antibody - BSA Free

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Protocols

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FAQs

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