ER beta/NR3A2 Antibody (7B10.7) - Azide and BSA Free

Novus Biologicals | Catalog # NBP1-41194

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Chemotaxis

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7B10.7

Format

Azide and BSA Free
View all ER beta/NR3A2 Primary Antibodies »

Product Specifications

Immunogen

Amino acids 1-153 of human ER beta/NR3A2 expressed in E. coli.

Localization

Nuclear

Specificity

This antibody will recognize human ER beta.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ER beta/NR3A2 Antibody (7B10.7) - Azide and BSA Free

Western Blot: ER beta/NR3A2 Antibody (7B10.7) [NBP1-41194]

Western Blot: ER beta/NR3A2 Antibody (7B10.7) [NBP1-41194]

Western Blot: ER beta/NR3A2 Antibody (7B10.7) [NBP1-41194] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody [7B10.7]. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for ER beta/NR3A2 Antibody (7B10.7) - Azide and BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1-5 ug/ml
Application Notes
ChIP usage reported in scientific literature. WB: Predicted MW is 59 kDa.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ER beta/NR3A2

The human ER-beta is a newly discovered estrogen receptor initially cloned and characterized from testis. The size and structure of ER-beta is very similar to ER-alpha with the ligand and DNA binding domains being highly conserved, while the amino terminus which serves as their transactivation domain has diverged significantly. Similar in function to ER-alpha ER-beta binds to estrogen with a high affinity and regulates estrogen specific gene activation through direct interaction with estrogen response elements (ERE's).

Long Name

Estrogen Receptor beta

Alternate Names

ESR2, NR3A2

Entrez Gene IDs

2100 (Human)

Gene Symbol

ESR2

Additional ER beta/NR3A2 Products

Product Documents for ER beta/NR3A2 Antibody (7B10.7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ER beta/NR3A2 Antibody (7B10.7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ER beta/NR3A2 Antibody (7B10.7) - Azide and BSA Free

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FAQs

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail