ERAB Antibody

Novus Biologicals | Catalog # NB300-887

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Peptide ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ERAB Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence CIRLDGAIRMQP corresponding to C-Terminus according to NP_004484.1, NP_001032900.1.

Reactivity Notes

Mouse reactivity determined by Western Blot reported in scientific literature (PMID: 17436268).

Specificity

This antibody is expected to recognise both reported isoforms.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ERAB Antibody

Western Blot: ERAB Antibody [NB300-887]

Western Blot: ERAB Antibody [NB300-887]

Western Blot: ERAB Antibody [NB300-887] - Analysis of ERAB / HADH2 in Human Brain lysate (RIPA buffer, 30ug total protein per lane) using this antibody at 0.3ug/ml. Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Applications for ERAB Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 2 ug/ml
Application Notes
WB: Approx. 25 kDa band observed in human brain lysates (calculated MW of 26.9 kDa band according to NP_004484). A minor band of unknown identity was also consistently observed at appr 38 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ERAB

This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II, a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids, alcohols, and steroids. The protein has been implicated in the development of Alzheimer's disease, and mutations in the gene are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined.

Alternate Names

17-beta-HSD 10, 17-beta-hydroxysteroid dehydrogenase 10, 17b-HSD10, 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, ABAD, amyloid-beta peptide binding alcohol dehydrogenase, CAMR, DUPXp11.22, EC 1.1.1.178, EC 1.1.1.35, Endoplasmic reticulum-associated amyloid beta-peptide-binding protein, ERAB3-hydroxyacyl-CoA dehydrogenase type-2, HADH2AB-binding alcohol dehydrogenase, hydroxyacyl-Coenzyme A dehydrogenase, type II, hydroxyacyl-Coenzyme Adehydrogenase, type II, hydroxysteroid (17-beta) dehydrogenase 10, mental retardation, X-linked, syndromic 10, MHBD, Mitochondrial ribonuclease P protein 2,3-hydroxyacyl-CoA dehydrogenase type II, Mitochondrial RNase P protein 2, MRPP2HCD2, MRX17, MRX31, MRXS10, SCHAD, SDR5C1, short chain dehydrogenase/reductase family 5C, member 1, short chain L-3-hydroxyacyl-CoA dehydrogenase type 2, short chain type dehydrogenase/reductase XH98G2, Short-chain type dehydrogenase/reductase XH98G2, Type II HADH, XH98G2

Entrez Gene IDs

3028 (Human); 15108 (Mouse); 63864 (Rat)

Gene Symbol

HSD17B10

UniProt

NP_004484. (Human)

Additional ERAB Products

Product Documents for ERAB Antibody

Certificate of Analysis

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Product Specific Notices for ERAB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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