ERP29 Antibody

Novus Biologicals | Catalog # NBP1-05028

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Peptide with sequence C-EKNKMSDGKKEELQ corresponding to C-Terminus according to NP_006808.1.

Epitope

C-Terminus

Marker

Endoplasmic Reticulum Marker

Specificity

This antibody is expected to recognize isoform 1 (NP_006808.1) only.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ERP29 Antibody

Western Blot: ERP29 Antibody [NBP1-05028]

Western Blot: ERP29 Antibody [NBP1-05028]

Western Blot: ERP29 Antibody [NBP1-05028] - (0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for ERP29 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Peptide ELISA

Detection limit 1:2000

Western Blot

0.1-0.3ug/ml
Application Notes
WB: Approx. 28 kDa band observed in human liver lysates (calculated MW of 29.0 kDa band according to NP_006808.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ERP29

This gene encodes a reticuloplasmin, a protein which resides in the lumen of the endoplasmic reticulum (ER). The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Alternate Names

C12orf8chromosome 12 open reading frame 8, endoplasmic reticulum lumenal protein ERp28, endoplasmic reticulum protein 29, Endoplasmic reticulum resident protein 28, endoplasmic reticulum resident protein 29, ERp28Endoplasmic reticulum resident protein 31, ERp29, ERp31ERP28, PDIA9, PDI-DB, protein disulfide isomerase family A, member 9

Entrez Gene IDs

10961 (Human); 67397 (Mouse); 117030 (Rat)

Gene Symbol

ERP29

Additional ERP29 Products

Product Documents for ERP29 Antibody

Certificate of Analysis

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Product Specific Notices for ERP29 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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