ERP29 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-88396
![Immunohistochemistry-Paraffin: ERP29 Antibody [NBP1-88396]](https://resources.rndsystems.com/images/products/ERP29-Antibody-Immunohistochemistry-Paraffin-NBP1-88396-img0008.jpg "Immunohistochemistry-Paraffin: ERP29 Antibody [NBP1-88396]")
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Key Product Details
Validated by
Orthogonal Validation, Independent Antibodies
Species Reactivity
Validated:
Human, Rat
Predicted:
Mouse (97%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ALPLDTVTFYKVIPKSKFVLVKFDTQYPYGEKQDEFKRLAENSASSDDLLVAEVGISDYGDKLNMELSEKYKLD
Marker
Endoplasmic Reticulum Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ERP29 Antibody - BSA Free
![Immunohistochemistry-Paraffin: ERP29 Antibody [NBP1-88396]](https://resources.rndsystems.com/images/products/ERP29-Antibody-Immunohistochemistry-Paraffin-NBP1-88396-img0010.jpg "Immunohistochemistry-Paraffin: ERP29 Antibody [NBP1-88396]")
Immunohistochemistry-Paraffin: ERP29 Antibody [NBP1-88396]
Immunohistochemistry-Paraffin: ERP29 Antibody [NBP1-88396] - Staining of human cerebral cortex, lymph node, prostate and skeletal muscle using Anti-ERP29 antibody NBP1-88396 (A) shows similar protein distribution across tissues to independent antibody NBP1-88395 (B).![Western Blot: ERP29 Antibody [NBP1-88396]](https://resources.rndsystems.com/images/products/ERP29-Antibody-Western-Blot-NBP1-88396-img0011.jpg "Western Blot: ERP29 Antibody [NBP1-88396]")
![Immunohistochemistry-Paraffin: ERP29 Antibody [NBP1-88396]](https://resources.rndsystems.com/images/products/ERP29-Antibody-Immunohistochemistry-Paraffin-NBP1-88396-img0015.jpg "Immunohistochemistry-Paraffin: ERP29 Antibody [NBP1-88396]")
Immunohistochemistry-Paraffin: ERP29 Antibody [NBP1-88396]
Immunohistochemistry-Paraffin: ERP29 Antibody [NBP1-88396] - Staining of human skeletal muscle using Anti-ERP29 antibody NBP1-88396.![Western Blot: ERP29 Antibody [NBP1-88396]](https://resources.rndsystems.com/images/products/ERP29-Antibody-Western-Blot-NBP1-88396-img0009.jpg "Western Blot: ERP29 Antibody [NBP1-88396]")
Western Blot: ERP29 Antibody [NBP1-88396]
Western Blot: ERP29 Antibody [NBP1-88396] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.![Immunohistochemistry-Paraffin: ERP29 Antibody [NBP1-88396]](https://resources.rndsystems.com/images/products/ERP29-Antibody-Immunohistochemistry-Paraffin-NBP1-88396-img0006.jpg "Immunohistochemistry-Paraffin: ERP29 Antibody [NBP1-88396]")
Immunohistochemistry-Paraffin: ERP29 Antibody [NBP1-88396]
Immunohistochemistry-Paraffin: ERP29 Antibody [NBP1-88396] - Staining of human prostate shows high expression.![Immunohistochemistry-Paraffin: ERP29 Antibody [NBP1-88396]](https://resources.rndsystems.com/images/products/ERP29-Antibody-Immunohistochemistry-Paraffin-NBP1-88396-img0012.jpg "Immunohistochemistry-Paraffin: ERP29 Antibody [NBP1-88396]")
Immunohistochemistry-Paraffin: ERP29 Antibody [NBP1-88396]
Immunohistochemistry-Paraffin: ERP29 Antibody [NBP1-88396] - Staining of human lymph node.![Immunohistochemistry-Paraffin: ERP29 Antibody [NBP1-88396]](https://resources.rndsystems.com/images/products/ERP29-Antibody-Immunohistochemistry-Paraffin-NBP1-88396-img0013.jpg "Immunohistochemistry-Paraffin: ERP29 Antibody [NBP1-88396]")
Immunohistochemistry-Paraffin: ERP29 Antibody [NBP1-88396]
Immunohistochemistry-Paraffin: ERP29 Antibody [NBP1-88396] - Staining of human cerebral cortex.![Immunohistochemistry-Paraffin: ERP29 Antibody [NBP1-88396]](https://resources.rndsystems.com/images/products/ERP29-Antibody-Immunohistochemistry-Paraffin-NBP1-88396-img0014.jpg "Immunohistochemistry-Paraffin: ERP29 Antibody [NBP1-88396]")
Immunohistochemistry-Paraffin: ERP29 Antibody [NBP1-88396]
Immunohistochemistry-Paraffin: ERP29 Antibody [NBP1-88396] - Staining of human prostate using Anti-ERP29 antibody NBP1-88396.![ERP29 Antibody - BSA Free Immunohistochemistry-Paraffin: ERP29 Antibody - BSA Free [NBP1-88396]](https://resources.rndsystems.com/images/products/nbp1-88396_rabbit-polyclonal-erp29-antibody-6520251617122.jpg "Immunohistochemistry-Paraffin: ERP29 Antibody - BSA Free [NBP1-88396]")
Immunohistochemistry-Paraffin: ERP29 Antibody - BSA Free [NBP1-88396]
Staining of human skeletal muscle shows low expression as expected.Applications for ERP29 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ERP29
Alternate Names
C12orf8chromosome 12 open reading frame 8, endoplasmic reticulum lumenal protein ERp28, endoplasmic reticulum protein 29, Endoplasmic reticulum resident protein 28, endoplasmic reticulum resident protein 29, ERp28Endoplasmic reticulum resident protein 31, ERp29, ERp31ERP28, PDIA9, PDI-DB, protein disulfide isomerase family A, member 9
Entrez Gene IDs
10961 (Human)
Gene Symbol
ERP29
UniProt
Additional ERP29 Products
Product Documents for ERP29 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ERP29 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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