Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 801 and 851 of human ABT1-Associated Protein using the numbering given in entry NP_057733.2 (GeneID 51575).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ESF1 Antibody - BSA Free
Immunoprecipitation: ESF1 Antibody [NBP1-71920]
Immunoprecipitation: ESF1 Antibody [NBP1-71920] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-ABTAP antibody used for WB at 0.1 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. ABTAP was also immunoprecipitated by another rabbit anti-ABTAP antibody, which recognizes an upstream epitope.Applications for ESF1 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: ESF1
Alternate Names
ABT1-associated protein, ABTAP, bA526K24.1, C20orf6, chromosome 20 open reading frame 6, ESF1 homolog, ESF1, nucleolar pre-rRNA processing protein, homolog (S. cerevisiae), FLJ20368
Gene Symbol
ESF1
UniProt
Additional ESF1 Products
Product Documents for ESF1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for ESF1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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