Ethenoadenosine Antibody (1G4) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80727

Novus Biologicals
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Key Product Details

Species Reactivity

All Species

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2 Lambda Clone # 1G4

Format

Azide and BSA Free
View all Ethenoadenosine Primary Antibodies »

Product Specifications

Immunogen

Ethenoadenosine

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2 Lambda

Scientific Data Images for Ethenoadenosine Antibody (1G4) - Azide and BSA Free

Flow Cytometry: Ethenoadenosine Antibody (1G4) - Azide and BSA Free [NBP2-80727]

Flow Cytometry: Ethenoadenosine Antibody (1G4) - Azide and BSA Free [NBP2-80727]

Flow Cytometry: Ethenoadenosine Antibody (1G4) - Azide and BSA Free [NBP2-80727] - Detection of ADP-ribosylated proteins by flow cytometry. C-33A cells (1 × 107/ml), transiently transfected with a Flag-tagged chicken ART4 containing plasmid, were incubated at 37C in the presence or absence of 200 uM etheno-NAD+ for 30 min. After washing, cells were stained with an etheno-adenosine specific antibody (1G4). Image collected and cropped by CiteAb from the following publication (https://bmcmolbiol.biomedcentral.com/articles/10.1186/1471-2199-9-86), licensed under a CC-BY license. Image from the standard format of this antibody.
Flow Cytometry: Ethenoadenosine Antibody (1G4) - Azide and BSA Free [NBP2-80727]

Flow Cytometry: Ethenoadenosine Antibody (1G4) - Azide and BSA Free [NBP2-80727]

Flow Cytometry: Ethenoadenosine Antibody (1G4) - Azide and BSA Free [NBP2-80727] - Chicken erythrocytes (1% v/v), isolated by density gradient centrifugation, were transiently transfected with a Flag-tagged chicken ART4 containing plasmid or the empty plasmid (pSecTag B), and incubated at 37C in the presence or absence of 200 uM etheno-NAD+ for 30 min. After washing, cells were stained with an etheno-adenosine specific antibody (1G4) or an anti-Flag antibody and the respective isotype control stained with the 1G4 antibody. Image collected and cropped by CiteAb from the following publication (https://bmcmolbiol.biomedcentral.com/articles/10.1186/1471-2199-9-86), licensed under a CC-BY license. Image from the standard format of this antibody.
Flow Cytometry: Ethenoadenosine Antibody (1G4) - Azide and BSA Free [NBP2-80727]

Flow Cytometry: Ethenoadenosine Antibody (1G4) - Azide and BSA Free [NBP2-80727]

Flow Cytometry: Ethenoadenosine Antibody (1G4) - Azide and BSA Free [NBP2-80727] - Etheno-ADP-ribosylation of cell-surface proteins on C3H-10T1/2 cells. C3H-10T 1/2 cells at different stages of differentiation (progenitors, MyoD-myoblasts, myotubes) were incubated for 30 minutes in the absence (dashed lines) or presence (solid lines) of 100 uM etheno-NAD. Cells were then washed, incubated with the primary monoclonal antibody 1G4, stained with a FITC-conjugated goat-anti-mouse secondary antibody and subjected to FACS analysis. Data show one representative experiments out of three. Image collected and cropped by CiteAb from the following publication (https://bmcmolbiol.biomedcentral.com/articles/10.1186/1471-2199-9-91), licensed under a CC-BY license. Image from the standard format of this antibody.

Applications for Ethenoadenosine Antibody (1G4) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000. Use reported in scientific literature (PMID 9774627)

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunoprecipitation

1:10-1:500

Western Blot

1:800
Application Notes
This antibody is CyTOF ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ethenoadenosine

ADP-ribosylation represents a posttranslational modification that involves transfer of ADP-ribose moiety from NAD+ to a specific amino acid in target protein (greatly modifying protein function) with the release of nicotinamide moiety. It was originally discovered as a mechanism by which potent bacterial toxins, such as Diphtheria toxin and Cholera toxin, modify and inactivate human target proteins, and these toxins were suggested to mimic mammalian endogenous ADP-ribosyltransferases. ART2 is an ecto-ADP-ribosyltransferases family (ART1-ART5) member which distantly resembles ADP-ribosylating bacterial toxins. It is expressed by mature T cells in which it ADP-ribosylates the integrin LFA-1 as well as other cell surface proteins, and this ART2-mediated ADP-ribosylation of T cell surface proteins provides a novel means for triggering T cell apoptosis. Ethenoadenosine moiety is contained in NAD analogue etheno-NAD which serves as an efficient substrate for ADP-ribosyltransferases and ethenoadenosine specific antibody clone 1G4 can be used for detection of ADP-ribosylated amino acids (ADP-ribosylation of cellular proteins) by FACS/FLOW, ICC-IF and Western blot applications.

Alternate Names

C12H13N5O4, N6-Ethenoadenosine

Additional Ethenoadenosine Products

Product Documents for Ethenoadenosine Antibody (1G4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Ethenoadenosine Antibody (1G4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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