ExCellerate™ T Cell Media, Animal Component-Free
R&D Systems | Catalog # CCM035
Key Product Details
Features
Key Benefits
- Phenol red-free formulation in 1 L bottles
- Robust T cell expansion under serum-free and animal component-free conditions
- Optimized for use with R&D Systems cytokines and G-Rex® bioreactors
- Compatible with a variety of T cell activation methods
- Supports T cell expansion from PBMCs or purified CD3+ T cells
Species
Product Summary for ExCellerate™ T Cell Media, Animal Component-Free
ExCellerate T Cell Media is optimized for T cell expansion in a G-Rex bioreactor. This media is compatible with a variety of cytokine combinations (IL-2, IL-7, and IL-15) AF RUO Proteins from R&D Systems in different cell activation methods, including particle, bead-based, or plate-bound. The activation reagents, culture vessel, and cytokine growth factor combinations used with this media should be optimized by application or experimental protocol.
Scientific Data Images for ExCellerate™ T Cell Media, Animal Component-Free
Stem Cell Memory Population is enhanced when using ExCellerate™ Human T Cell Media, ACF compared to other media suppliers.
Two donors were evaluated after nine days in culture within a G-Rex® Bioreactor with media supplemented with 10 ng/mL of BT007-AFL and 10 ng/mL of BT-015-AFL. Flow Cytometry was used to evaluate CCR7+ T Cells.A fully Animal-Free and Serum-Free T Cell process expands viable T cells similar to other Xeno-Free media alternatives.
Two donors were evaluated for expansion and viability during a 9 day culture period in a G-Rex® bioreactor supplemented with 10 ng/mL of BT-007-AFL and 10 ng/mL of BT-015-AFL.Excellerate™ Human T Cell Media, Animal-Component Free supports edited and non-edited T cell cultures. Both Lentiviral transduction and TcBuster™ transposition of a CD19 CAR was evaluated at day 8 and 9 of culture within a G-Rex® bioreactor.
Both Lentiviral transduction and TcBuster™ transposition of a CD19 CAR was evaluated at day 8 and 9 of culture within a G-Rex® bioreactor.Kit Contents for ExCellerate™ T Cell Media, Animal Component-Free
1 L Bottle of ExCellerate T Cell Media, Animal Component-FreeFormulation, Preparation, and Storage
Shipping
Storage
Stability
Product Documents for ExCellerate™ T Cell Media, Animal Component-Free
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for ExCellerate™ T Cell Media, Animal Component-Free
FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
- The safety and efficacy of this product in diagnostic or other clinical uses has not been established.
- This reagent should not be used beyond the expiration date indicated on the label.
- Results may vary due to variations among cells derived from different donors.
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