ExCellerate™ T Cell Media, Animal Component-Free

R&D Systems | Catalog # CCM035

This media has been discontinued. Please see CCM038-GMP for cell therapy applications and CCM030 for basic T cell research applications.
R&D Systems
Discontinued Product
CCM035 has been discontinued. An alternative/replacement product is available: CCM038-GMP-1L. View all Primary Cell Culture products.

Key Product Details

Features

This media has been discontinued. Please see CCM038 for cell therapy applications and see CCM030 for basic T cell research applications.

Key Benefits

  • Phenol red-free formulation in 1 L bottles
  • Robust T cell expansion under serum-free and animal component-free conditions
  • Optimized for use with R&D Systems cytokines and G-Rex® bioreactors
  • Compatible with a variety of T cell activation methods
  • Supports T cell expansion from PBMCs or purified CD3+ T cells

Species

Human

Product Summary for ExCellerate™ T Cell Media, Animal Component-Free

ExCellerate T Cell Media is formulated and optimized for the ex vivo culture of human T lymphocytes. Unlike traditional serum-containing culture media, animal component-free media provides a stable culture and optimized environment that facilitates the generation and expansion of T cells. The medium supports expansion of routine culture of human T cells while maintaining high viability and expansion of stem cell memory T Cells.
Versatility of ExCellerate T Cell Media, Animal Component-Free

ExCellerate T Cell Media is optimized for T cell expansion in a G-Rex bioreactor. This media is compatible with a variety of cytokine combinations (IL-2, IL-7, and IL-15) AF RUO Proteins from R&D Systems in different cell activation methods, including particle, bead-based, or plate-bound. The activation reagents, culture vessel, and cytokine growth factor combinations used with this media should be optimized by application or experimental protocol.

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Scientific Data Images for ExCellerate™ T Cell Media, Animal Component-Free

Stem Cell Memory Population is enhanced when using ExCellerate™ Human T Cell Media, ACF compared to other media suppliers.

Two donors were evaluated after nine days in culture within a G-Rex® Bioreactor with media supplemented with 10 ng/mL of BT007-AFL and 10 ng/mL of BT-015-AFL. Flow Cytometry was used to evaluate CCR7+ T Cells.

A fully Animal-Free and Serum-Free T Cell process expands viable T cells similar to other Xeno-Free media alternatives.

Two donors were evaluated for expansion and viability during a 9 day culture period in a G-Rex® bioreactor supplemented with 10 ng/mL of BT-007-AFL and 10 ng/mL of BT-015-AFL.

Excellerate™ Human T Cell Media, Animal-Component Free supports edited and non-edited T cell cultures. Both Lentiviral transduction and TcBuster™ transposition of a CD19 CAR was evaluated at day 8 and 9 of culture within a G-Rex® bioreactor.

Both Lentiviral transduction and TcBuster™ transposition of a CD19 CAR was evaluated at day 8 and 9 of culture within a G-Rex® bioreactor.

Kit Contents for ExCellerate™ T Cell Media, Animal Component-Free

1 L Bottle of ExCellerate T Cell Media, Animal Component-Free

Formulation, Preparation, and Storage

Shipping

The product is shipped at 2-8 °C. Upon receipt, store immediately at the recommended temerature. Protect from light.

Storage

Store the unopened product at 2-8 °C protected from light. Do not use past expiration date.

Stability

Product is stable for 12 months from date of manufacture when stored at 2-8 °C. See lot specific Certificate of Analysis for expiration date.

Product Documents for ExCellerate™ T Cell Media, Animal Component-Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for ExCellerate™ T Cell Media, Animal Component-Free

FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

  • The safety and efficacy of this product in diagnostic or other clinical uses has not been established.
  • This reagent should not be used beyond the expiration date indicated on the label.
  • Results may vary due to variations among cells derived from different donors.

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