ExCellerate Human T Cell Expansion Media, Xeno-Free New
ExCellerate Human T Cell Expansion Media, Xeno-Free Summary
Media for the ex vivo expansion and culture of human T cell lymphocytes under xeno-free conditions.
- Robust T cell expansion under serum-free and xeno-free conditions
- Optimized for use with the Cloudz™ T Cell Activation Kit
- Compatible with a variety of T cell activation methods
- Supports T cell expansion from PBMCs or purified CD3+ T cells
Why Use ExCellerate Human T Cell Expansion Media?
T Lymphocytes (T Cells) are a critical component of the adaptive immune response. While dysregulation of T cell function and proliferation contributes to the etiology of many diseases, the ability of T cells to activate an immune response toward specific antigens is being harnessed as a powerful immunotherapy tool to combat cancer and other diseases. To facilitate discovery and preclinical research for T cell immunoregulation and therapy, robust platforms for ex vivo expansion and maintenance of T cells, including specialized serum-free or xeno-free media, are required.
ExCellerate Human T Cell Expansion Media is formulated and optimized for the ex vivo culture of human T lymphocytes in research applications. Unlike traditional serum-containing culture media, xeno-free media provides a stable culture and optimized environment, void of non-human animal-derived products, that facilitates the generation and expansion of T cells. The medium supports expansion of routine culture of human T cell lines or T cell clones and the stimulation of human peripheral blood lymphocytes.
Versatility of ExCellerate Human T Cell Expansion Media.
ExCellerate Human T Cell Expansion Media is optimized for T cell expansion when used in combination with Cloudz T Cell Activation Kit and Recombinant Human Cytokines from R&D Systems. However, this versatile media also provides robust growth using a variety of cytokine combinations (IL-2, IL-7, and IL-15) and cell activation methods, including particle, bead-based, or plate-bound anti-CD3/anti-CD28 antibodies. The activation and cytokine/growth factor combinations used with this media should be optimized by application or experimental protocol.
- 500 mL of ExCellerate Human T Cell Expansion Media
Fold Expansion of Human T Cells in ExCellerate Human T Cell Expansion Media. Primary human peripheral blood mononuclear cells (PBMCs) were cultured for 9 days in ExCellerate Human T Cell Expansion Media using the Cloudz T Cell Activation Kit and Recombinant Human IL-2 (R&D Systems, Catalog # 202-IL). Cell counts were performed to determine fold expansion compared to the Day 0 seeding density (0.25 x 106 cells/mL).
Phenotypic Analysis of Human T cells Expanded in Xeno-Free Human T Cell Expansion Media. Human PBMCs were cultured for 9 days in ExCellerate Human T Cell Expansion Media using the Cloudz T Cell Activation Kit and Recombinant Human IL-2 (R&D Systems, Catalog # 202-IL). Human T Cells were enriched during the culture as indicated via the expression of positive T cell markers, including Mouse anti-Human CD3 epsilon APC-conjugated Antibody (Catalog # FAB100A) and with a Mouse Anti-Human CD8 alpha PE-conjugated Antibody (Catalog # FAB1509).
Preparation and Storage
Background: Hematopoietic Stem Cells
HSC Differentiation &
The definitive hematopoietic system is made up of all adult blood cell types including megakaryocytes, erythrocytes, and cells of the myeloid and lymphoid lineages. All of these cells are derived from multipotent hematopoietic stem cells (HSCs) through a succession of precursors with progressively limited potential. Hematopoietic stem cells are tissue-specific stem cells that exhibit remarkable self-renewal capacity and are responsible for the life-long maintenance of the hematopoietic system. HSCs are rare cells that reside in adult bone marrow where hematopoiesis is continuously taking place. They can also be found in cord blood, fetal liver, adult spleen, and peripheral blood. R&D Systems offers several products for studying hematopoietic lineage cells including serum-free media, lineage depletion antibodies and kits, and reagents for performing colony forming cell (CFC) assays.
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