Exosome Detection (Simple Western) Antibody Pack
Novus Biologicals | Catalog # NBP3-11742
![Simple Western: Exosome Detection (Simple Western) Antibody Pack [NBP3-11742]](https://resources.rndsystems.com/images/products/Exosome-Detection-Simple-Western-Antibody-Pack-Simple-Western-NBP3-11742-img0001.jpg "Simple Western: Exosome Detection (Simple Western) Antibody Pack [NBP3-11742]")
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Key Product Details
Species
Human, Mouse, Rat
Applications
Flow Cytometry, Immunohistochemistry, Simple Western, Western Blot
Product Summary for Exosome Detection (Simple Western) Antibody Pack
This pack contains 1 vial each of: MAB50482-SP (25 ug); NBP2-67884 (100 uL); MAB1663-SP (25 ug); NB100-1965SS (0.025 ml), HAF007 (1 mL), HAF008 (1 ml)
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Product Specifications
Specificity
Please see each vial's data sheet for specificity information specific to each product.
Application Notes
See individual datasheets of components for their validated applications
Reactivity Notes
See individual datasheets of components for their validated species
Scientific Data Images for Exosome Detection (Simple Western) Antibody Pack
Simple Western: Exosome Detection (Simple Western) Antibody Pack [NBP3-11742]
Simple Western: Exosome Detection (Simple Western) Antibody Pack [NBP3-11742] - Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) by heat shocked (HS), loaded at 0.2 mg/mL. A specific band was detected for HSP70/HSPA1A at approximately 67 kDa (as indicated) using 0.5 ug/mL of Mouse Anti-Human/Mouse/Rat HSP70/HSPA1A Monoclonal Antibody (Catalog # MAB1663). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.![Simple Western: Exosome Detection (Simple Western) Antibody Pack [NBP3-11742]](https://resources.rndsystems.com/images/products/Exosome-Detection-Simple-Western-Antibody-Pack-Simple-Western-NBP3-11742-img0002.jpg "Simple Western: Exosome Detection (Simple Western) Antibody Pack [NBP3-11742]")
Simple Western: Exosome Detection (Simple Western) Antibody Pack [NBP3-11742]
Simple Western: Exosome Detection (Simple Western) Antibody Pack [NBP3-11742] - Simple Western lane view shows lysates of human platelets, loaded at 0.2 mg/mL. A specific band was detected for CD63 at approximately 59 kDa (as indicated) using 20 ug/mL of Rabbit Anti-Human CD63 Monoclonal Antibody (Catalog # MAB50482). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Kit Contents for Exosome Detection (Simple Western) Antibody Pack
- MAB1663-SP
- MAB50482-SP
- HAF007: Mouse IgG Horseradish Peroxidase-conjugated Antibody
- NB100-1965
- NBP2-67884
- HAF008: Rabbit IgG Horseradish Peroxidase-conjugated Antibody
Formulation, Preparation, and Storage
Formulation
See individual datasheets
Concentration
Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Storage is content dependent.
Background: Exosome Detection (Simple Western)
Additional Exosome Detection (Simple Western) Products
Product Documents for Exosome Detection (Simple Western) Antibody Pack
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Exosome Detection (Simple Western) Antibody Pack
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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