F-Actin Antibody (NH3) - BSA Free

Novus Biologicals | Catalog # NBP2-61610

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Chicken, Rabbit

Applications

ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Kappa Clone # NH3

Format

BSA Free
View all F-Actin Primary Antibodies »

Product Specifications

Immunogen

Human monocytes and the human monocyte-like cell line, U937.

Specificity

The epitope recognized is preserved across species (mouse, rabbit and human) and for different isoforms. NH3 recognises human filamentous actin (F-actin), but has been reported to recognise globular actin (G-actin).

Clonality

Monoclonal

Host

Mouse

Isotype

IgM Kappa

Applications for F-Actin Antibody (NH3) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Thiophilic Chromatography

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: F-Actin

In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.

Alternate Names

ACT, ACTG, actin, cytoplasmic 2, actin, gamma 1, BRWS2, DFNA20, DFNA26

Additional F-Actin Products

Product Documents for F-Actin Antibody (NH3) - BSA Free

Certificate of Analysis

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Product Specific Notices for F-Actin Antibody (NH3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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